Active Recombinant Human IFNG Protein

• Cat.No.: IFNG-99H
• Product Overview: Recombinant Human Interferon gamma is produced by our E.coli expression system and the target gene encoding Gln24-Gln166 is expressed.

Specification

• Description: IFNγ is the major interferon produced by mitogenically or antigenically stimulated lymphocytes. It is structurally different from type I interferon and its major activity is immunoregulation. It has been implicated in the expression of class II histocompatibility antigens in cells that do not normally produce them, leading to autoimmune disease. Interferon gamma is produced mainly byT-cells and natural killer cells activated by antigens, mitogens, or alloantigens. It is produced by lymphocytes expressing the surface antigens CD4 and CD8. IFNγ synthesis is induced by IL-2, FGF-basic, and EGF.
• Source: E. coli
• Species: Human
• Form: Lyophilized from a 0.2 μm filtered solution of 20mM Tris-HCl, 250mM NaCl, pH 8.5.
• Bio-activity: Measured by a cytotoxicity assay using HT-29 cells. The ED50 for this effect is 17 pg/mL.
• Protein Length: Gln24-Gln166
• AA Sequence: MQDPYVKEAENLKKYFNAGHSDVAD NGTLFLGILKNWKEESDRKIMQSQI VSFYFKLFKNFKDDQSI QKSVETIKEDMNVKFF NSNKKKRDDFEKLTNYSVTDLNVQR KAIHELIQVMAELSPAAKTGKRKRS QMLFRGRRASQ
• Endotoxin: Less than 0.1 ng/ug (1 EU/ug).
• Purity: >95%
• Storage: Lyophilized protein should be stored at < -20 centigrade, though stable at room temperature for 3 weeks.
  Reconstituted protein solution can be stored at 4-7 centigrade for 2-7 days.
  Aliquots of reconstituted samples are stable at < -20 centigrade for 3 months.
• Quality Statement: Purity: Greater than 95% as determined by reducing SDS-PAGE.
• Shipping: The product is shipped at ambient temperature.
  Upon receipt, store it immediately at the temperature listed below.

Gene Information

• Gene Name: IFNG interferon gamma [ Homo sapiens (human) ]
• Official Symbol: IFNG
• Synonyms: Interferon Gamma; IFN-Gamma; Immune Interferon; IFG; IFI; IMD69
• Gene ID: 3458
• mRNA Refseq: NM_000619.3
• Protein Refseq: NP_000610.2
• MIM: 147570
• UniProt ID: P01579