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Cat. No. |
ADAM17-592H |
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Product Overview |
Recombinant human
glycosylated catalytic domain (aa Pro1-Val477) of ADAM17/TACE
secreted as mature, active enzyme from insect cells, and purified
using a C-terminal His-tag, 36kDa. |
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Description |
ADAM17/TACE is a
soluble or membrane-bound metalloproteinase primarily responsible
for activation of proTNF-α, while also targeting proteins such as
fractalkine, amyloid precursor proteins, and CD40. ADAM17/TACE is
involved in cancer, vascular disorders, and inflammatory diseases
such as rheumatoid arthritis and focal ischemic injury. The
catalytic domain of ADAM17/TACE is able to cleave proTNF-α and can
be used in inhibitor screening. |
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Source |
insect cells. |
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Concentration |
0.45 µg/µl. |
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Purity |
≥90% (SDS-PAGE). |
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Formulation |
Liquid. In 22.5mM
TRIS, pH 7.5, containing 4.5µM ZnCl2, 0.0045% Brij-35 and 10%
glycerol. |
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Specific Activity:
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2’430 U/µg enzyme.
One unit will hydrolyze one pmole Mca-PLAQAV-Dpa-RSSSR-NH2 substrate
(10µM) per minute at 37oC, in 25mM TRIS, pH 9.0. |
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Application |
Study enzyme
kinetics, cleave target substrates, screen inhibitors. |
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Storage |
Avoid freeze/thaw
cycles. After opening, prepare aliquots and store at -80oC. |
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Gene Information
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Gene Name |
ADAM17 ADAM metallopeptidase domain 17 [ Homo sapiens ] |
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Synonyms
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ADAM
metallopeptidase domain 17; CSVP; TACE; ADAM18; CD156B; MGC71942;
ADAM17; disintegrin and metalloproteinase domain-containing protein
17; ADAM 17; EC 3.4.24.86; TNF-alpha convertase; snake venom-like
protease; TNF-alpha converting enzyme; TNF-alpha-converting enzyme;
ADAM metallopeptidase domain 18;tumor necrosis factor, alpha,
converting enzyme; a disintegrin and metalloprotease domain 17;
CD156b antigen |
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GeneID |
6668 |
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mRNA Refseq |
NM_003183 |
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Protein Refseq |
NP_003174 |
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MIM |
603639 |
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UniProt ID |
P78536 |
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Chromosome Location |
2p25 |
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Pathways |
Alzheimer"s
disease; Epithelial cell signaling in Helicobacter pylori infection;
Notch signaling pathway; Signaling by EGFR; Signalling by NGF |
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Function |
PDZ domain binding;
SH3 domain binding; integrin binding; interleukin-6 receptor
binding; metal ion binding; metalloendopeptidase activity;
metalloendopeptidase activity; metallopeptidase activity; peptidase
activity; protein binding; zinc ion binding |
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PDB rendering based on 1bkc
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