Recombinant Dog EPO Protein, His-tagged

• Cat.No.: EPO-904D
• Product Overview: Recombinant Dog EPO Protein (Ala41-Arg206) with His tag was expressed in E. coli.

Specification

• Description: This gene encodes a secreted, glycosylated cytokine composed of four alpha helical bundles. The encoded protein is mainly synthesized in the kidney, secreted into the blood plasma, and binds to the erythropoietin receptor to promote red blood cell production, or erythropoiesis, in the bone marrow. Expression of this gene is upregulated under hypoxic conditions, in turn leading to increased erythropoiesis and enhanced oxygen-carrying capacity of the blood. Expression of this gene has also been observed in brain and in the eye, and elevated expression levels have been observed in diabetic retinopathy and ocular hypertension. Recombinant forms of the encoded protein exhibit neuroprotective activity against a variety of potential brain injuries, as well as antiapoptotic functions in several tissue types, and have been used in the treatment of anemia and to enhance the efficacy of cancer therapies.
• Source: E. coli
• Species: Dog
• Tag: His
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 18.41 kDa
• Protein length: Ala41-Arg206
• Purity: > 97%
• Applications: Positive Control; Immunogen; SDS-PAGE; WB.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 centigrade for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 centigrade for one month. Aliquot and store at -80 centigrade for 12 months.
• Storage Buffer: PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
• Reconstitution: Reconstitute in PBS or others.

Gene Information

• Gene Name: EPO erythropoietin [ Canis lupus familiaris (dog) ]
• Official Symbol: EPO
• Synonyms: EPO; erythropoietin
• Gene ID: 404002
• mRNA Refseq: NM_001006646
• Protein Refseq: NP_001006647
• UniProt ID: P33707

Reviews

12/28/2021

Stability is truly remarkable, can maintain its activity even under extreme conditions.

08/31/2021

Can catalyze reactions even under extreme conditions.

02/03/2019

This protein's repeatability is truly exceptional, consistently delivering reliable results even under challenging conditions.

Q&As

What is the relationship between EPO protein and cardiovascular disease?

EPO protein can promote the renewal and repair of heart blood vessels, and has the effect of protecting the heart from ischemia-reperfusion damage.

How does EPO protein regulate erythropoiesis?

By binding to receptors, EPO proteins activate signaling pathways that promote the proliferation and differentiation of hematopoietic stem cells in bone marrow into erythrocytes.

How about the clinical use of EPO protein?

EPO protein can be used by injection or oral, and the specific method and dosage need to be determined according to the condition and the doctor's advice.

What are the implications of EPO protein research for the future of treating anemia?

The study of EPO protein helps to understand the regulatory mechanism of erythropoiesis and provides new ideas and methods for the treatment of anemia, such as developing more effective EPO protein analogues or improving existing treatment regimens.

How is EPO protein treated for anemia?

This protein stimulates the bone marrow to produce more red blood cells, thereby increasing hemoglobin levels and treating anemia.

What is the role of EPO protein in kidney disease?

In kidney disease, due to impaired kidney function, the production of EPO protein decreases, leading to anemia. Supplementation with exogenous EPO protein can help correct anemia caused by kidney problems.