Recombinant Human ADCYAP1 293 Cell Lysate

• Cat.No.: ADCYAP1-9019HCL

Specification

• Description: Antigen standard for adenylate cyclase activating polypeptide 1 (pituitary) (ADCYAP1), transcript variant 2 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid (True ORF cDNA clone RC210851, OriGene Technologies, Inc.) and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
• Source: HEK 293 cells
• Species: Human
• Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
• Size: 0.1 mg
• Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
• Applications: E, IP, WB. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.

Gene Information

• Gene Name: ADCYAP1 adenylate cyclase activating polypeptide 1 (pituitary) [ Homo sapiens ]
• Official Symbol: ADCYAP1
• Synonyms: ADCYAP1; adenylate cyclase activating polypeptide 1 (pituitary); pituitary adenylate cyclase-activating polypeptide; PACAP; MGC126852
• Gene ID: 116
• mRNA Refseq: NM_001099733
• Protein Refseq: NP_001093203
• MIM: 102980
• UniProt ID: P18509
• Chromosome Location: 18p11
• Pathway: Activation of TRKA receptors, organism-specific biosystem; Class B/2 (Secretin family receptors), organism-specific biosystem; G alpha (s) signalling events, organism-specific biosystem; GPCR downstream signaling, organism-specific biosystem; GPCR ligand binding, organism-specific biosystem; Glucagon-type ligand receptors, organism-specific biosystem; NGF signalling via TRKA from the plasma membrane, organism-specific biosystem
• Function: neuropeptide hormone activity; peptide hormone receptor binding; peptide hormone receptor binding; receptor binding; receptor signaling protein activity

Q&As

Is ADCYAP1 currently being used as a treatment for Alzheimer's disease?

ADCYAP1 is not currently being used as a treatment for Alzheimer's disease. However, there are ongoing studies investigating the use of ADCYAP1 and its analogues as potential therapeutics for the treatment of cognitive impairment in Alzheimer's disease and other neurodegenerative disorders.

How can ADCYAP1 be used in the treatment of inflammatory disorders?

ADCYAP1 can be used as a potential therapeutic target for the treatment of inflammatory disorders. Drugs that target ADCYAP1 could potentially inhibit the production of pro-inflammatory cytokines and increase the levels of anti-inflammatory cytokines, leading to a reduction in inflammation.

How can ADCYAP1 be used in the treatment of migraines?

ADCYAP1 can be used as a potential therapeutic target for the treatment of migraines. Drugs that target ADCYAP1 could potentially block its activity and reduce the release of neuropeptides like substance P and CGRP that are involved in migraine pain sensitization.

Are there any side effects associated with targeting ADCYAP1?

The potential side effects of targeting ADCYAP1 are currently unknown, as there are no drugs targeting this protein that have reached clinical trials. However, the complex regulation and interactions of ADCYAP1 with other systems, such as the immune system, may increase the risk of side effects. In addition, as ADCYAP1 plays a role in various physiological processes, targeting it may have unintended effects beyond the intended therapeutic target.

Are there any drugs currently targeting ADCYAP1?

There are currently no drugs targeting ADCYAP1 that have been approved by regulatory agencies for clinical use. However, there are ongoing preclinical studies investigating the use of ADCYAP1 and its analogues as potential therapeutics for various conditions, including Alzheimer's disease, diabetes, and inflammatory disorders.

What are some potential therapeutic applications of targeting ADCYAP1 protein?

Targeting ADCYAP1 protein may have therapeutic applications in various diseases and conditions, including migraine headache, Alzheimer's disease, schizophrenia, diabetes, and inflammatory disorders.

How is ADCYAP1 being targeted for drug development?

Various approaches are being explored for targeting ADCYAP1 protein in drug development, including small molecule inhibitors, monoclonal antibodies, and gene therapy. Small molecule inhibitors that target ADCYAP1 activity or expression are being developed and tested in preclinical studies. Monoclonal antibodies that bind to ADCYAP1 or its receptors are being developed for diagnostic and therapeutic purposes. Gene therapy approaches are being explored to deliver ADCYAP1-targeted therapeutics to specific tissues.

Are there any drugs in clinical trials that target ADCYAP1?

As of 2021, there are no drugs targeting ADCYAP1 that have reached clinical trials. However, several small molecule inhibitors of ADCYAP1 are being developed by pharmaceutical companies and tested in preclinical studies.

How is ADCYAP1 involved in inflammatory disorders?

ADCYAP1 has anti-inflammatory properties and has been shown to regulate the function of immune cells such as macrophages and T cells. It reduces inflammation by inhibiting the production of pro-inflammatory cytokines and increasing the levels of anti-inflammatory cytokines. Elevated levels of ADCYAP1 have been found in the synovial fluid of patients with rheumatoid arthritis, suggesting that it may play a role in modulating the inflammatory response in this disorder.

How can ADCYAP1 be used in the treatment of diabetes?

ADCYAP1 can be used as a potential therapeutic target for the treatment of diabetes. Drugs that target ADCYAP1 could potentially stimulate insulin release from pancreatic beta cells and enhance insulin sensitivity in peripheral tissues, leading to improved glucose homeostasis.

What are some potential biomarkers for monitoring the efficacy of ADCYAP1-targeted therapies?

As ADCYAP1 is involved in various physiological processes and diseases, potential biomarkers for monitoring the efficacy of ADCYAP1-targeted therapies may depend on the disease or condition being treated. For example, in migraine headache, biomarkers such as headache frequency and severity, medication use, and neuroimaging measures may be used. In Alzheimer's disease, biomarkers such as cognitive function and neuroimaging measures may be monitored. In diabetes, biomarkers such as blood glucose levels and insulin sensitivity may be measured.

How is ADCYAP1 involved in Alzheimer's disease?

ADCYAP1 has neuroprotective properties and has been shown to improve cognitive function in animal models of Alzheimer's disease. It also regulates the production and clearance of beta-amyloid, a protein that accumulates in the brains of Alzheimer's patients and is associated with neurodegeneration. However, studies have also shown that elevated levels of ADCYAP1 in the brain may contribute to the formation of tau protein, another hallmark of Alzheimer's disease pathology.

What are some challenges in developing ADCYAP1-targeted therapies?

One challenge in developing ADCYAP1-targeted therapies is that ADCYAP1 is not the only neuropeptide that plays a role in the physiological processes and diseases in which it is implicated. Its physiological effects may also depend on its interactions with other neuropeptides, receptors, and signaling pathways. Additionally, the complex regulation and interactions of ADCYAP1 with other systems, such as the immune system, may complicate the design and optimization of ADCYAP1-targeted therapeutics.