Recombinant Human ApoE3

• Cat.No.: ApoE3-3562H
• Product Overview: ApoE3

Specification

• Description: Chylomicron remnants and very low density lipoprotein (VLDL) remnants are rapidly removed from the circulation by receptor-mediated endocytosis in the liver. Apolipoprotein E, a main apoprotein of the chylomicron, binds to a specific receptor on liver cells and peripheral cells. ApoE is essential for the normal catabolism of triglyceride-rich lipoprotein constituents. The APOE gene is mapped to chromosome 19 in a cluster with APOC1 and APOC2. Defects in apolipoprotein E result in familial dysbetalipoproteinemia, or type III hyperlipoproteinemia (HLP III), in which increased plasma cholesterol and triglycerides are the consequence of impaired clearance of chylomicron and VLDL remnants.
• Source: E. Coli
• Species: Human
• Form: Lyophilized
• Molecular Mass: 36,154 Da
• Protein length: 317
• Purity: >90% by SDS-PAGE & HPLC analyses
• Notes: Small volumes of ApoE3 recombinant protein may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice.
• Warning: This product is for research use only. Not for use in diagnostic or therapeutic procedures.

Gene Information

• Gene Name: ApoE3
• Official Symbol: ApoE3
• Synonyms: APOE; APOE; AD2; LPG; LDLCQ5; Apo-E
• Gene ID: 348
• mRNA Refseq: NM_000041.2
• Protein Refseq: NP_000032.1
• MIM: 104310
• UniProt ID: P02649
• Chromosome Location: Chromosome: 19; NC_000019.10 (44905782..44909393). Location: 19q13.2
• Function: ?ApoE is essential for the normal catabolism of triglyceride-rich lipoprotein constituents. The APOE gene is mapped to chromosome 19 in a cluster with APOC1 and APOC2. Defects in apolipoprotein E result in familial dysbetalipoproteinemia, or type III hyperlipoproteinemia (HLP III), in which increased plasma cholesterol and triglycerides are the consequence of impaired clearance of chylomicron and VLDL remnants

Reviews

10/07/2022

By using ApoE3 protein, I can explore the mechanisms underlying cell division, cell cycle control, and the molecular pathways associated with these processes.

07/03/2019

This proactive approach not only aids in expanding my knowledge but also encourages continuous improvement and innovation in my experimental approach.

03/06/2018

As a researcher utilizing ApoE3 protein in my trials, I can highlight its advantages and the valuable support provided by the manufacturer in facilitating my research.

07/05/2017

The protein's interaction with other APC/C subunits enables the study of its function and the investigation of its involvement in various cellular processes.

Q&As

Does the ApoE3 protein have any role in inflammation or immune response?

The ApoE3 protein has been shown to have anti-inflammatory properties and can modulate immune responses. It has been suggested that ApoE3 may have a protective effect against neuroinflammation and certain autoimmune diseases, although further research is needed to fully understand these mechanisms.

Can the ApoE3 protein interact with lipids other than cholesterol?

Yes, the ApoE3 protein can interact with various lipids besides cholesterol, including triglycerides and phospholipids. Its ability to bind to different types of lipids contributes to its role in lipid metabolism and transport.

Can the ApoE3 protein be used in gene therapy or genetic engineering?

The ApoE3 protein itself is not typically used in gene therapy or genetic engineering applications. However, knowledge about the structure and function of the ApoE3 protein can be utilized in gene therapy approaches targeting the ApoE gene to regulate lipid metabolism or address specific conditions.

Can the ApoE3 protein be studied to understand disease risk and develop potential therapies?

While the ApoE3 protein itself may not be directly targeted for therapeutic interventions, studying its functions and variations in the context of ApoE genotypes can help researchers understand disease risk factors and develop potential therapies for conditions associated with other ApoE isoforms, such as ApoE4 and Alzheimer's disease.

Are there any known drug interactions specific to the ApoE3 protein?

There are no known drug interactions specific to the ApoE3 protein. However, it is important for healthcare professionals to consider an individual's ApoE genotype when prescribing certain medications, as the presence of the ApoE4 allele may impact drug metabolism or response.

Does the ApoE3 genotype affect cognitive function or memory?

The ApoE3 genotype is generally considered to have a neutral effect on cognitive function and memory compared to the ApoE4 genotype, which is a major risk factor for late-onset Alzheimer's disease. However, individual variations and other genetic and environmental factors can still influence an individual's cognitive abilities.

Are there any ongoing research studies focused on the ApoE3 protein?

Current biomedical research often emphasizes the ApoE4 isoform due to its strong association with Alzheimer's disease. Nevertheless, studies that investigate the functions and implications of the ApoE3 protein continue to contribute to our understanding of lipid metabolism, cardiovascular health, and other aspects related to the ApoE gene.

How does the ApoE3 protein affect brain health and neurodegenerative diseases?

The ApoE3 protein is generally considered to have a neutral or beneficial effect on brain health compared to the ApoE4 protein, which is associated with an increased risk of neurodegenerative diseases like Alzheimer's. However, individual factors and genetic interactions can influence the role of ApoE3 in brain health and disease.

Can genetic testing determine if an individual has the ApoE3 allele?

Yes, genetic testing can determine whether an individual has the ApoE3 allele. The ApoE gene can be analyzed through genetic testing methods, such as DNA sequencing or genotyping, to determine an individual's ApoE genotype and the presence of the ApoE3 allele.

How does the ApoE3 protein differ from the other ApoE isoforms?

The primary difference between ApoE3 and the other ApoE isoforms (ApoE2 and ApoE4) lies in their amino acid sequences. ApoE3 is characterized by the presence of the cysteine residue at positions 112 and 158 of the protein, which distinguishes it from ApoE4 (cysteine at 112 and arginine at 158) and ApoE2 (cysteine at 112 and cysteine at 158).

Can the ApoE3 protein influence the risk of Alzheimer's disease?

The ApoE3 protein is not strongly associated with an increased or decreased risk of Alzheimer's disease. People with the ApoE3 isoform have a relatively average risk compared to those with the other ApoE isoforms (ApoE2 and ApoE4).

Can the ApoE3 protein be implicated in cardiovascular diseases?

The ApoE3 protein is less associated with cardiovascular diseases compared to ApoE4, which has a stronger correlation with increased cardiovascular risks. Nevertheless, it is essential to maintain a healthy lifestyle and manage cardiovascular risk factors regardless of ApoE genotype.

Can the ApoE3 protein be targeted for therapeutic interventions?

The ApoE3 protein itself is not a specific target for therapeutic interventions. However, understanding the role of ApoE isoforms, including ApoE3, in lipid metabolism and disease risk can inform research on potential treatments and interventions for diseases associated with other ApoE isoforms.

Does the ApoE3 protein have any known effects on cognition or brain function?

The ApoE3 protein is generally considered to have no significant effects on cognition or brain function. It is not strongly associated with cognitive decline or an increased risk of neurodegenerative diseases like Alzheimer's disease.

How is the ApoE3 protein linked to lipid metabolism?

The ApoE3 protein participates in lipid metabolism by interacting with lipoprotein particles. It serves as a ligand for various receptors, facilitating the transport and redistribution of lipids throughout the body. The ApoE3 isoform's balanced binding properties contribute to its neutral role in lipid metabolism compared to other ApoE isoforms.

Can the ApoE3 allele increase the risk of other diseases apart from Alzheimer's?

In general, the ApoE3 allele is not strongly associated with increased susceptibility to specific diseases like Alzheimer's or other neurodegenerative disorders. However, it is important to note that some complex diseases may have multifactorial causes involving interactions between genetic and environmental factors, which can influence disease risk even with a relatively neutral ApoE3 allele.

How does the ApoE3 protein impact lipid transport and metabolism in the brain?

The ApoE3 protein plays a crucial role in lipid transport and metabolism in the brain. It is produced by astrocytes and binds to lipoprotein particles, which are then taken up by neurons through receptors. This process is important for the maintenance of brain lipid homeostasis.

Does the ApoE3 protein have any known protective effects?

While the ApoE3 protein does not confer the same protective effects as the ApoE2 isoform (which is associated with a decreased risk of Alzheimer's disease), it is considered a relatively neutral isoform regarding disease risk. It does not show a significant association with increased susceptibility to various disorders like atherosclerosis or neurodegeneration.

Can the ApoE3 protein be inherited from both parents?

Yes, the ApoE3 allele can be inherited from both parents. Each individual has two copies of the ApoE gene, one inherited from each parent. If an individual inherits one ApoE3 allele from one parent and another ApoE3 allele from the other parent, they will have the ApoE3/3 genotype.

Is there any ongoing research to uncover potential therapeutic interventions targeting the ApoE3 protein?

Researchers continue to investigate various aspects of ApoE biology, including ApoE3, to understand its role in disease processes and explore potential therapeutic interventions. Although therapies targeting ApoE3 specifically may be less common, the knowledge gained from these studies can contribute to broader advancements in disease treatment and prevention.

Are there any specific medical recommendations for individuals with the ApoE3 allele?

In general, there are no specific medical recommendations exclusively for individuals with the ApoE3 allele. However, maintaining overall health through lifestyle factors like a balanced diet, regular exercise, and managing cardiovascular risks is recommended for everyone, including those with the ApoE3 allele.

How does the ApoE3 protein function in the body?

Like other ApoE isoforms, the ApoE3 protein plays a role in lipid metabolism. It is involved in the transport and redistribution of lipids, particularly cholesterol, in the body. It interacts with receptors on cells to regulate the uptake and utilization of lipoproteins.

Can the ApoE3 protein be used as a therapeutic target for specific diseases?

While the ApoE3 protein itself may not be directly targeted for therapies, researchers explore various strategies to modulate the effects of different ApoE isoforms, including ApoE3, for therapeutic purposes. These approaches aim to regulate lipid metabolism, reduce pathological processes, and potentially address diseases associated with altered ApoE function.

Can lifestyle factors modify the risk associated with the ApoE3 allele?

Lifestyle factors, such as diet, exercise, and overall health maintenance, can potentially modify disease risk for individuals with the ApoE3 allele. Engaging in a healthy lifestyle can help mitigate other risk factors and promote overall well-being, regardless of specific genetic variations.