Recombinant Human ARSF 293 Cell Lysate

• Cat.No.: ARSF-8675HCL

Specification

• Description: Antigen standard for arylsulfatase F (ARSF) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
• Source: HEK 293 cells
• Species: Human
• Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
• Size: 0.1 mg
• Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
• Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.

Gene Information

• Gene Name: ARSF arylsulfatase F [ Homo sapiens ]
• Official Symbol: ARSF
• Synonyms: ARSF; arylsulfatase F; ASF
• Gene ID: 416
• mRNA Refseq: NM_001201538
• Protein Refseq: NP_001188467
• MIM: 300003
• UniProt ID: P54793
• Chromosome Location: Xp22.3
• Function: arylsulfatase activity; hydrolase activity; metal ion binding

Reviews

09/14/2022

Its reliability and effectiveness in generating accurate data make it an indispensable resource, driving scientific exploration and advancing our understanding of protein structure and function

02/26/2017

Researchers can rely on the ARSF protein to deliver precise results, enhancing the credibility and efficiency of their experiments.

03/09/2016

By incorporating the ARSF protein into electron microscopy studies, researchers gain insights into the intricate three-dimensional architecture of proteins, facilitating a deeper comprehension of their functions and aiding in the development of novel therapeutics.

Q&As

Are there any known interactions between the ARSF protein and other molecules?

The ARSF protein may interact with various molecules within the cellular environment. For example, it has been reported to interact with other sulfatases, transporters of sulfate-containing molecules, and certain signaling proteins. These interactions can regulate its activity or contribute to different physiological processes and pathways involving sulfate metabolism.

Are there any therapeutic strategies being explored for ARSF-related disorders?

Therapeutic strategies for ARSF-related disorders such as CDPX2 are still limited. However, some approaches are being explored, including gene therapy, enzyme replacement therapy, and small molecule modulators to restore or enhance ARSF protein function. These strategies hold promise but require further research and development.

How does the ARSF protein differ from other sulfatase enzymes?

The ARSF protein belongs to the sulfatase enzyme family, which includes several other members involved in sulfate metabolism. Each sulfatase enzyme functions on different substrates and has distinct tissue distribution. While the ARSF protein shares similarities with other sulfatases, it has its specific characteristics and plays a unique role in the hydrolysis of certain sulfate esters.

Can the ARSF protein be used as a diagnostic or prognostic marker for certain diseases?

Currently, the ARSF protein is not widely used as a specific diagnostic or prognostic marker for diseases beyond its association with CDPX2. However, as research progresses and our understanding of the ARSF protein expands, it is possible that it may be utilized in the future for diagnostic, prognostic, or even therapeutic purposes in certain diseases or conditions.

What are the potential roles of the ARSF protein beyond sulfate metabolism?

While the ARSF protein is primarily known for its involvement in sulfate metabolism, emerging research suggests it may have additional functions. Some studies have suggested that ARSF may play a role in cell adhesion and migration processes, potentially influencing tissue development and repair. Further research is needed to fully understand these additional roles.

Are there any known diseases or conditions associated with ARSF protein dysregulation?

Currently, the primary disorder associated with ARSF protein dysregulation is X-linked chondrodysplasia punctata type 2 (CDPX2). This disorder results from mutations in the ARSF gene, leading to deficient or impaired ARSF protein activity. However, there may be other diseases or conditions that involve ARSF dysregulation, and ongoing research continues to investigate its potential involvement in various disorders.

How is ARSF protein deficiency diagnosed?

ARSF protein deficiency, specifically CDPX2, can be diagnosed through genetic testing. A blood or saliva sample is usually collected to analyze the DNA and identify mutations in the ARSF gene. Additionally, clinical evaluation and radiographic imaging may be used to confirm the diagnosis.

Is there a treatment available for ARSF protein deficiency or CDPX2?

Currently, there is no specific cure for ARSF protein deficiency or CDPX2. Treatment is usually focused on managing the symptoms and complications associated with the condition. This may include interventions such as physical therapy for skeletal abnormalities, cataract surgery, and supportive care for intellectual disability and other associated issues.

What happens when the ARSF protein is mutated or dysfunctional?

Mutations in the ARSF gene can lead to a deficiency or reduced activity of the ARSF protein. This can result in a condition called chondrodysplasia punctata, specifically X-linked chondrodysplasia punctata type 2 (CDPX2). CDPX2 is characterized by skeletal abnormalities, cataracts, intellectual disability, and other features.