Recombinant Human CA12, His-tagged
Cat.No. : | CA12-104H |
Product Overview : | Recombinant human CA12, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques. |
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Description : | CA12, also known as Carbonic anhydrase 12, is an enzyme that in humans is encoded by the CA12 gene. Carbonic anhydrases(CAs) are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. This gene product is a type I membrane protein that is highly expressed in normal tissues, such as kidney, colon and pancreas, and has been found to be overexpressed in 10% of clear cell renal carcinomas. |
Source : | Insect cell |
Species : | Human |
Tag : | His |
Form : | Liquid. In phosphate buffered saline (pH7.4) containing 10% glycerol. |
Molecular Mass : | 31.94kDa (283aa)(28-40kDa in SDS-PAGE under reducing conditions.) |
AA Sequence : | APVNGSKWTY FGPDGENSWS KKYPSCGGLL QSPIDLHSDI LQYDASLTPL EFQGYNLSAN KQFLLTNNGH SVKLNLPSDM HIQGLQSRYSATQLHLHWGN PNDPHGSEHT VSGQHFAAEL HIVHYNSDLY PDASTASNKS EGLAVLAVLI EMGSFNPSYD KIFSHLQHVK YKGQEAFVPGFNIEELLPER TAEYYRYRGS LTTPPCNPTV LWTVFRNPVQ ISQEQLLALE TALYCTHMDD PSPREMINNF RQVQKFDERL VYTSFSQVQVCTAAGLSHHH HHH |
Endotoxin : | < 1.0 eu per 1 microgram of protein (determined by lal |
Purity : | >90% by SDS - PAGE |
Storage : | Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles. |
Concentration : | 0.5mg/ml (determined by absorbance at 280nm) |
Gene Name : | CA12 carbonic anhydrase XII [ Homo sapiens ] |
Official Symbol : | CA12 |
Synonyms : | CA12; carbonic anhydrase XII; carbonic anhydrase 12; HsT18816; CA-XII; carbonic dehydratase; carbonate dehydratase XII; tumor antigen HOM-RCC-3.1.3; CAXII; FLJ20151; |
Gene ID : | 771 |
mRNA Refseq : | NM_001218 |
Protein Refseq : | NP_001209 |
MIM : | 603263 |
UniProt ID : | O43570 |
Chromosome Location : | 15q22 |
Function : | carbonate dehydratase activity; lyase activity; metal ion binding; zinc ion binding; |
Products Types
◆ Recombinant Protein | ||
CA12-423R | Recombinant Rhesus Macaque CA12 Protein, His (Fc)-Avi-tagged | +Inquiry |
CA12-261H | Recombinant Human CA12 protein(Met1-Gln291), His-tagged | +Inquiry |
CA12-1455H | Recombinant Human CA12 protein, His-tagged | +Inquiry |
CA12-478H | Recombinant Human CA12 Protein, His (Fc)-Avi-tagged | +Inquiry |
CA12-1456C | Recombinant Cynomolgus CA12 protein, His-tagged | +Inquiry |
◆ Lysates | ||
CA12-3067MCL | Recombinant Mouse CA12 cell lysate | +Inquiry |
CA12-3061HCL | Recombinant Human CA12 cell lysate | +Inquiry |
Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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Q&As (7)
Ask a questionKnown inhibitors can be identified through drug screening assays, and their impact on CA12 can be studied using kinetic studies and cell-based assays.
Cellular localization can be identified using immunofluorescence, subcellular fractionation, or electron microscopy.
CA12 has been implicated in tumor progression, particularly in hypoxic environments, by modulating pH and thus influencing tumor cell survival and metastasis. This can be assessed using cellular assays and tumor models.
Downstream pathways can be identified using pathway analysis tools, phosphoproteomics, and specific signal transduction assays.
The activity modulation under different pH conditions can be determined using enzymatic assays and pH shift experiments.
Expression changes in response to stressors can be analyzed using techniques like qPCR, Western blotting, or proteomics following cellular treatment with specific agents.
Genetic variants and their impact can be determined using structural models and functional assays comparing wild-type and mutant proteins.
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