Recombinant Human CD274 Protein, His-tagged
Cat.No. : | CD274-199C |
Product Overview : | Recombinant cynomolgus monkey PD-L1 (Phe19-Thr239) protein fused with Fc region of human IgG1 (Pro100-Lys330) at C-terminus was expressed in human embryonic kidney cell HEK293. |
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Description : | B7-H1, also known as PD-L1 and CD274, is an approximately 65 kDa transmembrane glycoprotein in the B7 family of immune regulatory molecules. Mature cynomolgus B7-H1 consists of a 220 amino acid (aa) extracellular domain (ECD) with two immunoglobulin-like domains, a 21 aa transmembrane segment, and a 30 aa cytoplasmic domain. Within the ECD, cynomolgus B7-H1 shares 92%, 72%, and 72% aa sequence identity with human, mouse, and rat B7-H1, respectively. In addition, cynomolgus B7-H1 shares 98%, 94%, 94%, 88%, 78%, 98%, 95%, 94%, and 94% aa sequence identity with rhesus macaque, chimpanzee, sumatran orangutan, white-tufted-ear marmoset, Garnett's greater bushbaby, olive baboon, green monkey, western lowland gorilla, and northern white-cheeked gibbon B7-H1, respectively. B7-H1 is expressed on inflammatory-activated immune cells including macrophages, T cells, and B cells, keratinocytes, endothelial and intestinal epithelial cells, as well as a variety of carcinomas and melanoma. B7-H1 is a B7 ligand and binds to B7-1/CD80 and PD-1 receptors on T cells. It suppresses T cell activation and proliferation and induces the apoptosis of activated T cells. It plays a role in the development of immune tolerance by promoting T cell anergy and enhancing regulatory T cell development. B7-H1 favors the development of anti-inflammatory IL-10 and IL-22 producing dendritic cells and inhibits the development of Th17 cells. In cancer, B7-H1 provides resistance to T cell mediated lysis, enhances EMT, and enhances the tumorigenic function of Th22 cells. B7-H1/PD-1 coinhibitory pathway was exploited therapeutically resulting in remarkable outcomes with 20-90% response in various types of cancer. |
Source : | HEK293 |
Species : | Cynomolgus Monkey |
Tag : | Fc |
Form : | Disulfide-linked homodimer |
Molecular Mass : | 52 kDa |
N-terminal Sequence Analysis : | Phe19 |
Endotoxin : | <0.10 EU per 1 μg of the protein by the LAL method. |
Purity : | >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining. |
Storage : | Avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 centigrade as supplied. 1 month, 2 to 8 centigrade under sterile conditions after reconstitution. 3 months, -20 to -70 centigrade under sterile conditions after reconstitution. |
Storage Buffer : | Lyophilized from a 0.2 μm filtered solution in PBS. |
Reconstitution : | Reconstitute at 200 μg/mL in PBS. |
Protein length : | Phe19-Thr239 |
Gene Name : | CD274 CD274 molecule [ Macaca fascicularis (crab-eating macaque) ] |
Official Symbol : | CD274 |
Synonyms : | B7-H; B7H1; B7-H1; B7H1PDCD1L1; CD274 antigenMGC142294; CD274 molecule; CD274; PDCD1L1; PDCD1LG1; PDCD1LG1MGC142296; PDL1; PD-L1; PD-L1B7 homolog 1; PDL1PDCD1 ligand 1; programmed cell death 1 ligand 1; Programmed death ligand 1 |
Gene ID : | 102145573 |
mRNA Refseq : | XM_005581779 |
Protein Refseq : | XP_005581836 |
UniProt ID : | G7PSE7 |
Products Types
◆ Recombinant Protein | ||
Cd274-11M | Recombinant Mouse Cd274 Protein, His (Fc)-Avi-tagged | +Inquiry |
CD274-6778H | Recombinant Human CD274 Protein, His&T7-tagged | +Inquiry |
CD274-051H | Active Recombinant Human CD274 protein(Met1-Thr239), His&Avi-tagged, Biotinylated | +Inquiry |
CD274-054H | Recombinant Human CD274 Protein, His-tagged | +Inquiry |
CD274-4367R | Recombinant Rabbit CD274 Protein | +Inquiry |
◆ Lysates | ||
CD274-914CCL | Recombinant Cynomolgus CD274 cell lysate | +Inquiry |
CD274-002RCL | Recombinant Rat CD274 cell lysate | +Inquiry |
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For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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