Recombinant Human Fibroblast Growth Factor 18

• Cat.No.: FGF18-171H
• Product Overview: This recombinant human FGF-18 is a 21.2 kDa protein containing 182 amino acid residues and it was expressed inE.Coli.

Specification

• Cat. No.: FGF18-171H
• Description: Fibroblast Growth Factor-18 (FGF-18) is a heparin binding growth factor that stimulates the proliferation and activation of cells that express FGF receptors.
• Source: E.Coli.
• Supplied: Sterile filtered and the the protein was lyophilized from 10mM Tris, pH8.0 (no additives).
• Purity: >95% by SDS-Page and HPLC analyses. Endotoxin level is less than 0.1ng per ug (1EU/ug) of FGF-18.
• Reconstitution: The lyophilized FGF-18 should be reconstituted in 5mM TRIS, pH8.0 to a concentration of 0.1-1.0mg.ml. Allow to set at least 30 minutes at 4°C, tighten cap and rotate to reconstitute any material on sides of vial. If desired, you can centrifuge at 1000 rpm for 1 minute to concentrate volume in bottom of vial. This solution can be diluted into water or other buffered solutions and stored at 4°Cfor up to 1 week or stored at – 20℃ for future use. For most in-vitro applications, human FGF-18 exerts its biological activity in the concentration range of 0.1 to 20ng/ml.
• Note: If this material is not to be used for coating or labeling(or other applications requiring carrier free material), the addition of 0.1% BSA to dilution buffers or dilution in sterile culture media is recommended to enhance stability and minimize absorption to vial. Ideally, maintain concentration greater than 10ug/ml.
• Reactivity: The biological activity was determined by the does- dependent stimulation of thymidine uptake by BaF3 cells expressing FGF receptors yielding an ED50 <0.5ng/ml, corresponding to a specific activity of >1 X 106 (1 million) units/mg.
• Amino Acid Sequence: MAEENVDFRI HVENQTRARD DVSRKQLRLY QLYSRTSGKH IQVLGRRISA RGEDGDKYAQ LLVETDTFGS QVRIKGKETE FYLCMNRKGK LVGKPDGTSK ECVFIEKVLE NNYTALMSAK YSGWYVGFTK KGRPRKGPKT RENQQDVHFM KRYPKGQPE LQKPFKYTTV TKRSRRIRP THPAAHFIKR LYQGQLPFP NHAEKQKQFE FVGSAPTRRTK RTRR PQPLT
• Storage: The lyophilized powder is stable at room temperature for a few weeks but it is best stored desiccated at -20°C. Reconstituted human FGF-18 should be stored in working aliquots at -20°C.
• Tag: Non

Gene Information

• Gene Name: FGF18 fibroblast growth factor 18 [ Homo sapiens ]
• Synonyms: FGF18; fibroblast growth factor 18; ZFGF5; FGF-18; FGF-18; zFGF5
• Gene ID: 8817
• mRNA Refseq: NM_003862
• Protein Refseq: NP_003853
• MIM: 603726
• UniProt ID: O76093
• Chromosome Location: 5q34
• Pathway: MAPK signaling pathway; Melanoma; Pathways in cancer; Regulation of actin cytoskeleton
• Function: growth factor activity

Reviews

01/09/2022

the FGF18 protein exhibits remarkable stability, enabling it to maintain its structural integrity and functionality throughout the experimental process.

05/30/2021

The FGF18 protein offers exceptional quality and purity, which is crucial for ensuring reliable and reproducible experimental outcomes.

12/08/2017

As a researcher utilizing the FGF18 protein, I am fortunate to benefit from its numerous advantages in trials.

Q&As

What are the potential side effects of FGF18 therapy?

While generally considered safe, potential side effects may include local reactions at the injection site. Comprehensive studies are ongoing to assess long-term safety.

Is FGF18 only applicable to bone-related conditions?

While its primary focus is on musculoskeletal conditions, FGF18's role in cellular growth and repair suggests potential applications in other medical fields.

How does FGF18 compare to other growth factors in terms of clinical applications?

Comparative studies are underway to evaluate the effectiveness and safety of FGF18 against other growth factors in various clinical scenarios.

Are there any ongoing clinical trials involving FGF18?

Yes, there are several clinical trials exploring the safety and efficacy of FGF18 in various musculoskeletal disorders and conditions.

How does FGF18 contribute to the treatment of osteoarthritis?

FGF18 has shown promise in promoting cartilage repair and regeneration, making it a potential candidate for the development of therapies for osteoarthritis.