Recombinant Human MB Protein, His-tagged

• Cat.No.: MB-2424H
• Product Overview: Recombinant Human MB Protein (Met1-Gly154) with a N-His tag was expressed in E. coli.

Specification

• Description: This gene encodes a member of the globin superfamily and is predominantly expressed in skeletal and cardiac muscles. The encoded protein forms a monomeric globular haemoprotein that is primarily responsible for the storage and facilitated transfer of oxygen from the cell membrane to the mitochondria. This protein also plays a role in regulating physiological levels of nitric oxide. Multiple transcript variants encoding distinct isoforms exist for this gene.
• Source: E. coli
• Species: Human
• Tag: His
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 20.9 kDa
  Accurate Molecular Mass: 21 kDa
• Protein length: Met1-Gly154
• Endotoxin: <1.0 EU per 1µg (determined by the LAL method).
• Purity: > 97%
• Applications: Positive Control; Immunogen; SDS-PAGE; WB.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 centigrade for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 centigrade for one month. Aliquot and store at -80 centigrade for 12 months.
• Storage Buffer: 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
• Reconstitution: Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Gene Information

• Gene Name: MB myoglobin [ Homo sapiens (human) ]
• Official Symbol: MB
• Synonyms: MB; myoglobin; PVALB; MGC13548
• Gene ID: 4151
• mRNA Refseq: NM_005368
• Protein Refseq: NP_005359
• MIM: 160000
• UniProt ID: P02144

Reviews

08/05/2020

Effective in pull-down assay.

07/01/2020

Perfect for my experiments.

07/26/2018

High-quality product, very satisfied.

Q&As

How does the structure of myoglobin facilitate its function in muscle tissues?

Myoglobin's structure, with a single heme group, allows it to bind oxygen tightly, facilitating its role in oxygen storage and release in muscle tissues.

Why is myoglobin considered a marker for muscle injury, especially in the context of myocardial infarction?

Myoglobin is released into the bloodstream when muscle cells are damaged. In the context of a myocardial infarction (heart attack), damaged heart muscle cells release myoglobin, making it a quick, albeit non-specific, marker for muscle injury.

How is myoglobin's affinity for oxygen influenced by environmental conditions?

Myoglobin's affinity for oxygen remains relatively constant across various environmental conditions, ensuring a steady oxygen supply for muscles even under stress.

Are there any known diseases or conditions associated with mutations or dysregulation of MB?

While mutations in the MB gene are rare, they can lead to myoglobinuria, a condition where myoglobin is present in the urine, often after intense muscle activity.

What are the clinical implications of elevated myoglobin levels in blood tests?

Elevated myoglobin levels in blood tests can indicate muscle injury, including myocardial infarction. However, since myoglobin is also found in skeletal muscles, it is often used in conjunction with other markers to diagnose heart attacks.

How does myoglobin differ from hemoglobin in terms of oxygen binding and release?

While both myoglobin and hemoglobin bind oxygen, myoglobin has a higher affinity for oxygen and does not exhibit cooperative binding. This allows myoglobin to release oxygen under conditions where hemoglobin cannot, such as during intense muscle activity.

What is the primary function of the MB gene and its encoded protein?

The MB gene encodes the protein myoglobin, which is responsible for storing and releasing oxygen in muscle cells, especially in conditions of high oxygen demand.