Recombinant Human PARP1 Protein

• Cat.No.: PARP1-59H
• Product Overview: Recombinant Human PARP1 Protein without tag was expressed in E. coli.

Specification

• Description: This gene encodes a chromatin-associated enzyme, poly(ADP-ribosyl)transferase, which modifies various nuclear proteins by poly(ADP-ribosyl)ation. The modification is dependent on DNA and is involved in the regulation of various important cellular processes such as differentiation, proliferation, and tumor transformation and also in the regulation of the molecular events involved in the recovery of cell from DNA damage. In addition, this enzyme may be the site of mutation in Fanconi anemia, and may participate in the pathophysiology of type I diabetes.
• Source: E. coli
• Species: Human
• Unit Definition: One unit of PARP incorporates 10 femtomoles of NAD onto immobilized histones in 30 minutes. High specific activity PARP is provided at 10 units/μL.
• Applications: Identification of inhibitors of PARP1 activity
  Quantitation of DNA Damage
  Investigation of PARP1 inactivation during apoptosis
  Western Blot Analysis
• Stability: The enzyme retains over 66 % of its activity after 24 hr at 37 centigrade.
• Storage: Store at -20 centigrade in a manual defrost freezer. For long-term storage, freeze in working aliquots at -80 centigrade. Avoid freeze-thaw cycles.
• Concentration: 10 units/μL
• Storage Buffer: 20 mM Tris-Cl (pH 8.0), 200 mM NaCl, 1 mM DTT, 0.1% Triton X-100, 50% glycerol, and 0.1 mg/ml BSA.

Gene Information

• Gene Name: PARP1 poly(ADP-ribose) polymerase 1 [ Homo sapiens (human) ]
• Official Symbol: PARP1
• Synonyms: PARP1; poly(ADP-ribose) polymerase 1; PARP; PPOL; ADPRT; ARTD1; ADPRT1; PARP-1; ADPRT 1; pADPRT-1; poly [ADP-ribose] polymerase 1; ADP-ribosyltransferase (NAD+; poly (ADP-ribose) polymerase); ADP-ribosyltransferase NAD(+); ADP-ribosyltransferase diphtheria toxin-like 1; DNA ADP-ribosyltransferase PARP1; NAD(+) ADP-ribosyltransferase 1; poly (ADP-ribose) polymerase family, member 1; poly(ADP-ribose) synthetase; poly(ADP-ribosyl)transferase; poly[ADP-ribose] synthase 1; protein poly-ADP-ribosyltransferase PARP1; EC 2.4.2.30
• Gene ID: 142
• mRNA Refseq: NM_001618
• Protein Refseq: NP_001609
• MIM: 173870
• UniProt ID: P09874

Reviews

06/01/2020

This range of applications of PARP1 is breathtaking, and it can play an important role in any field.

12/02/2019

This short half-life and high clearance of PARP1 make it have greater potential in the treatment of tumors, infections and other diseases, and it is expected to become a new generation of therapeutic drugs.

03/18/2019

The purity and bioactivity of PARP1 make it a valuable resource for scientific research.

Q&As

Does PARP1 protein cause cells to die in time?

Yes, overactivation of the PARP1 protein can lead to apoptosis or necrosis of cells, which may be exploited in the treatment of certain diseases.

How is the PARP1 protein involved in DNA repair?

The PARP1 protein can repair DNA damage by attaching ADP-ribose groups to damaged DNA strands and recruiting other repair proteins.

What are the other regulatory mechanisms of PARP1 protein in DNA damage repair?

In addition to PARP1's involvement in direct DNA repair, it can also modulate the selection of repair pathways and initiate other DNA damage sensing mechanisms.

What is the potential of PARP1 protein in tumor immunotherapy?

Some studies have proposed that the interaction of PARP1 protein with DNA damage in tumor immunotherapy may affect the efficacy of immunotherapy.

Is PARP1 protein involved in cell cycle regulation?

Yes, the PARP1 protein is involved in the normal regulation of the cell cycle, especially G1/S and G2/M switching during DNA damage.

What are the effects of overactivation of PARP1 protein on cells?

Overactivation of PARP1 depletes energy stores such as NAD+ and ATP and leads to apoptosis, or cell death.