Recombinant Human TGFB1 Protein(Leu30-Arg278(C33S)), His-tagged

• Cat.No.: TGFB1-3753H
• Product Overview: Recombinant Human TGFB1 Protein(Leu30-Arg278(C33S))(P01137) is expressed from HEK293 with His tag at the N-Terminus.

Specification

• Source: HEK293
• Species: Human
• Tag: His
• Protein length: Leu30-Arg278(C33S)
• Form: Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized Human LAP (TGF beta 1) , His Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human ITGAV&ITGB6, His Tag with the EC50 of 1.0μg/ml determined by ELISA.
• Molecular Mass: The protein has a predicted MW of 31.4 kDa. Due to glycosylation, the protein migrates to 38-48 kDa based on Tris-Bis PAGE result.
• Endotoxin: Less than 1EU per μg by the LAL method.
• Purity: > 95% as determined by Tris-Bis PAGE
• Storage: -20 to -80°C for 12 months as supplied from date of receipt.
  -80°C for 3-6 months after reconstitution.
  2-8°C for 2-7 days after reconstitution.
  Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
• Reconstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

Gene Information

• Gene Name: TGFB1 transforming growth factor, beta 1 [ Homo sapiens ]
• Official Symbol: TGFB1
• Synonyms: TGFB1; transforming growth factor, beta 1; DPD1, TGFB; transforming growth factor beta-1; Camurati Engelmann disease; CED; TGFbeta; TGF-beta-1; TGF-beta 1 protein; latency-associated peptide; LAP; DPD1; TGFB
• Gene ID: 7040
• mRNA Refseq: NM_000660
• Protein Refseq: NP_000651
• MIM: 190180
• UniProt ID: P01137

Reviews

11/16/2021

The impressive performance of the TGFB1 protein both in ELISA assays and protein electron microscopy structure analysis makes it a highly versatile and recommended choice for scientists across various disciplines.

03/01/2020

Its reliability and efficacy in generating high-quality data make it an indispensable tool in the quest for unraveling the mysteries of protein structure and function.

07/16/2018

The TGFB1 protein's excellent performance in ELISA ensures accurate and reproducible results, enhancing the reliability of experimental data.

Q&As

How is TGFB1 involved in bone remodeling?

TGFB1 stimulates the activity of cells involved in bone formation (osteoblasts) and plays a role in regulating bone resorption by influencing osteoclast function.

Are there any side effects associated with therapies targeting TGFB1?

Potential side effects of anti-TGFB1 therapies may include immune system suppression, gastrointestinal issues, and skin reactions.

Can TGFB1 be used as a biomarker for certain diseases?

TGFB1 levels have been studied as potential biomarkers for diseases like cancer and fibrosis, aiding in diagnosis and monitoring disease progression.

In what ways does TGFB1 influence wound healing?

TGFB1 plays a critical role in wound healing by stimulating the formation of new blood vessels, promoting the production of extracellular matrix, and aiding in the migration of cells to the wound site.

How does TGFB1 contribute to fibrosis?

TGFB1 can induce the production of excessive extracellular matrix proteins, leading to tissue scarring and fibrosis in organs such as the liver, lung, and kidneys.