Recombinant Mouse ACCN3 Protein Pre-coupled Magnetic Beads

• Cat.No.: ACCN3-246M-B
• Product Overview: The Recombnant protein was conjugated to magnetic beads. This ready-to-use, pre-coupled magnetic beads are in uniform particle size and narrow size distribution with large surface area, which is conducive to convenient and fast capture target molecules with high specificity and achieve magnetic separation. This product can be equipped with automation equipment for high-throughput operations.

Specification

• Source: HEK293
• Species: Mouse
• Form: Solution
• Particle size: ~2 μm
• Beads Surface: Hydrophilic
• Capacity: > 200 pmol rabbit IgG/ mg beads
• Applications: Immunoassay, In vitro diagnostics, cell sorting, Immunoprecipitation/Co-precipitation, Protein/antibody separation and purification.
• Stability: Stable for at least 6 months from the date of receipt of the product under proper storage and handling conditions.
• Storage: 2-8℃. Do not to freeze thaw the Beads
• Concentration: 10mg beads/mL
• Storage Buffer: PBS buffer

Gene Information

• Gene Name: Accn3 amiloride-sensitive cation channel 3 [ Mus musculus ]
• Official Symbol: ACCN3
• Gene ID: 171209
• mRNA Refseq: NM_183000.2
• Protein Refseq: NP_892045.2
• UniProt ID: Q6X1Y6

Reviews

04/02/2023

I was hesitant to purchase the recombinant mouse ACCN3 protein at first, but I am glad I did. The quality was exceptional and the price was reasonable.

06/08/2020

I had some questions about the Recombinant Mouse ACCN3 Protein before purchasing and the customer service team was quick to provide answers.

02/06/2018

The technical support team was very helpful in assisting me with selecting the right fusion tag for the recombinant ACCN3 protein.

Q&As

What are the potential applications of the Recombinant ACCN3 Protein?

It may be useful for a range of applications, including biochemical and biophysical studies of its structure and function, drug screening and development targeting ACCN3, and in vitro diagnostic assays for pain or sensory disorders. The protein may also be used as an immunogen to generate specific antibodies against ACCN3 or as a standard in quantitative assays for protein detection.

What are the current approaches for studying ACCN3 function and regulation in experimental models?

Researchers employ various approaches to study ACCN3 function and regulation in experimental models. Electrophysiological techniques, such as patch-clamp recordings, allow direct measurement of ACCN3 channel activity and characterization of its biophysical properties. Pharmacological studies involve the use of specific ACCN3 agonists or antagonists to manipulate channel function and assess its physiological relevance. Genetic knockout or knockdown techniques in animal models provide valuable insights into the in vivo role of ACCN3 in sensory processing. Additionally, molecular biology and imaging techniques enable the investigation of ACCN3 expression patterns, protein-protein interactions, and intracellular signaling pathways involved in ACCN3 modulation.

What is the current understanding of the physiological functions of ACCN3?

ACCN3 is involved in multiple physiological processes related to sensory perception. It has been implicated in pain signaling, particularly in nociceptive neurons, where it plays a crucial role in detecting noxious stimuli. ACCN3 channels are also implicated in mechanosensation, contributing to the detection of mechanical pressure and touch. Additionally, ACCN3 participates in acid sensing, allowing neurons to respond to changes in extracellular pH.

Can the recombinant ACCN3 protein be used to generate antibodies for research purposes?

Yes, it can serve as an immunogen to generate specific antibodies for research purposes. The suitability of the protein for antibody production would depend on factors such as its antigenicity, purity, and stability.

How is ACCN3 regulated at the molecular level?

ACCN3 channels can be modulated by several mechanisms. One regulatory mechanism involves changes in pH: low extracellular pH can activate ACCN3 channels, leading to ion influx and cellular depolarization. ACCN3 activity can also be influenced by phosphorylation events. For example, protein kinase C (PKC) can phosphorylate ACCN3, modulating its activity. Furthermore, ACCN3 expression and function can be regulated by factors such as neuropeptides, neurotransmitters, and inflammatory mediators.

Can recombinant ACCN3 protein be used as a diagnostic or therapeutic agent?

Our recombinant protein products are primarily used as a research tool and are not approved for diagnostic or therapeutic purposes.

Are there any known post-translational modifications or alternative splicing variants of ACCN3 that should be considered?

It is essential to investigate whether ACCN3 undergoes any PTMs or has alternative splicing variants that might affect its activity or interactions. Reviewing the available literature or consulting databases such as UniProt or NCBI can provide insights into known ACCN3 PTMs or splice variants.

Is recombinant ACCN3 protein available in different forms, such as tagged and untagged?

Certainly, protein expression can be tailored according to your research needs. Modified versions can incorporate His tags, GST tags, or other fusion partners that aid in protein purification or detection.

Can the Recombinant ACCN3 Protein be used in co-immunoprecipitation or pull-down assays to identify interacting proteins?

Yes, by using ACCN3 as a bait, proteins that physically interact with ACCN3 can be captured and subsequently identified through mass spectrometry or immunoblotting techniques. It is recommended to optimize the experimental conditions, including appropriate controls and validation steps, to ensure the specificity and reliability of the identified interactions.

Do you provide any customization options for the Recombinant ACCN3 Protein, such as labeling or modifications?

Yes, and these options include protein labeling with fluorescent dyes or tags, or incorporation of specific modifications or mutations.