Recombinant Mouse AGO2 protein, His-tagged

• Cat.No.: AGO2-2241M
• Product Overview: Recombinant Mouse AGO2 protein(Q8CJG0)(Met1-Ala860), fused with His tag, was expressed in Insect Cells.

Specification

• Source: Insect Cells
• Species: Mouse
• Tag: His
• Form: Lyophilized from sterile 20mM Tris, 500mM NaCl, pH 7.4, 10% gly. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Molecular Mass: The recombinant mouse AGO2 consists of 878 amino acids and has a calculated molecular mass of 99 kDa. It migrates as an approximately 105 kDa band in SDS-PAGE under reducing conditions.
• Protein length: Met1-Ala860
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 80 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C
  Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Reviews

05/21/2021

The high purity of the protein product facilitated accurate quantification of target molecules.

10/08/2020

 Well-defined gel electrophoresis bands were indicative of the protein product’s pure form.

09/14/2017

 Incorporating the product led to a noticeable increase in target protein expression levels.

03/11/2017

 Precise results from immunoprecipitation assays were attributed to the protein product’s purity.

09/12/2016

Consistency across experiments made the protein product a reliable research tool.

Q&As

How is AGO2’s interaction with target mRNAs studied

Research explores how AGO2 guides small RNAs to complementary target mRNA sequences.

How is AGO2’s function studied in RNA silencing

Research explores AGO2’s role in guiding small RNAs to their target sequences.

Can AGO2’s activity be involved in antiviral defense

Yes, AGO2 is part of the antiviral RNA interference pathway.

Can AGO2 influence gene expression

Yes, AGO2’s activity leads to the suppression or degradation of target mRNAs.

How is AGO2’s involvement in chromatin remodeling studied

Research investigates AGO2’s connection to chromatin remodeling complexes.

How does AGO2’s dysfunction relate to diseases

AGO2 dysfunction is linked to disorders involving impaired gene regulation.

Is AGO2 essential for normal development

Yes, AGO2 is essential for normal development and cellular processes.

What’s the role of AGO2 in RNA interference

AGO2 plays a central role in mediating RNA silencing and post-transcriptional regulation.

How does AGO2’s role relate to post-transcriptional regulation

AGO2 helps control gene expression by degrading or inhibiting mRNA translation.

Can AGO2 influence stem cell maintenance

Yes, AGO2’s function is important for maintaining stem cell populations.

Is AGO2’s activity modulated during stress responses

Yes, AGO2’s activity can be regulated in response to cellular stress.

Can AGO2’s function be studied in cancer research

Yes, AGO2’s role in gene regulation is relevant in cancer studies.

Can AGO2 be involved in epigenetic regulation

Yes, AGO2’s role extends to epigenetic regulation through RNA-directed DNA methylation.

Can AGO2’s activity affect circadian rhythm regulation

Yes, AGO2’s function is linked to post-transcriptional regulation of circadian genes.

Can AGO2’s function impact heterochromatin formation

Yes, AGO2’s activity can influence heterochromatin formation and genome stability.

How does AGO2’s role in translation repression work

AGO2’s activity leads to translational repression through mRNA cleavage or inhibition.

Can AGO2 impact transposable element silencing

Yes, AGO2 is involved in suppressing transposable element activity.

How does AGO2 interact with small RNAs

AGO2 interacts with small RNAs, including microRNAs and small interfering RNAs.

How is AGO2’s role in exosome-mediated RNA decay studied

Research explores AGO2’s contribution to targeting RNAs for exosome-mediated decay.

How is AGO2’s involvement in germline development studied

Research investigates AGO2’s role in regulating germline development and fertility.

How does AGO2’s role relate to neuronal function

AGO2’s activity impacts neuronal development and synaptic plasticity.