Recombinant Rat Pdgfra Protein, His-tagged, Alexa Fluor 647 conjugated

• Cat.No.: Pdgfra-52RAF647
• Product Overview: Alexa Fluor 647 conjugated recombinant Rat Pdgfra (NP_036934.1) (Met1-Glu523), fused with a polyhistidine tag at the C-terminus, was produced in Human Cells.

Specification

• Source: HEK293
• Species: Rat
• Tag: His
• Form: Lyophilized
• Molecular Mass: The recombinant rat PDGFRA comprises 511 amino acids and predicts a molecular mass of 57.6 kDa. The apparent molecular mass of the recombinant protein is approximately 70-110 kDa in SDS-PAGE under reducing conditions.
• Protein length: 511
• N-terminal Sequence Analysis: Leu 24
• Endotoxin: < 1.0 EU/ μg of the protein as determined by the LAL method.
• Purity: > 95 % as determined by SDS-PAGE
• Characteristic: Disulfide-linked homodimer
  Labeled with Alexa Fluor 647 via amines
  Excitation = 650 nm
  Emission = 668 nm
• Stability: Samples are stable for up to 12 months from date of receipt at -70 centigrade.
• Storage: Store it under sterile conditions at -20 to -70 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Storage Buffer: Lyophilized from sterile PBS, pH 7.4. Normally 5%-8% trehalose and mannitol are added as protectants before lyophilization.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution. Centrifuge the vial at 4 centigrade before opening to recover the entire contents.

Gene Information

• Gene Name: Pdgfra platelet derived growth factor receptor, alpha polypeptide [ Rattus norvegicus ]
• Official Symbol: Pdgfra
• Gene ID: 25267
• mRNA Refseq: NM_012802
• Protein Refseq: NP_036934

Reviews

08/16/2022

The staff had a good attitude, actively communicated with me and solved many technical problems.

07/21/2022

Cheap price, high quality products, worth buying again!

02/04/2022

The high quality of the purchased protein helped me to obtain satisfactory experimental results.

Q&As

What are the effects of overexpressing PDGFRA?

Overexpression of PAGFRA promotes cancer cell proliferation and invasion and inhibits apoptosis.

What biological processes are PDGFRA involved in?

PDGFRA is involved in physiological processes such as normal organ development, tissue damage repair and malignant tumor progression.

What diseases has PDGFRA been closely associated with the study of?

PDGFRA is closely related to the study of gastrointestinal mesenchymal tumors, ovarian cancer and other diseases.

What is the relationship of PAGFRA to PDGF?

PDGFRA binds to PDGF to regulate cell proliferation and differentiation.