Recombinant Rat Sost protein(Met1-Tyr213), His-tagged

Cat.No. : Sost-3326R
Product Overview : Recombinant Rat SOST (NP_085073.1) (Met 1-Tyr 213) was expressed in HEK293, fused with a polyhistidine tag at the C-terminus.
Availability October 31, 2024
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Source : HEK293
Species : Rat
Tag : His
Protein length : Met1-Tyr213
Form : Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Bio-activity : Measured by its ability to inhibit Wnt3a induced alkaline phosphatase production by C3H10 T1/2 cells. The ED50 for this effect is typically 0.1-0.5 μg/mL.
Molecular Mass : The recombinant rat SOST comprises 201 amino acids and predicts a molecular mass of 23 kDa. The apparent molecular mass of the rat SOST is approximately 33 kDa in SDS-PAGE under reducing conditions due to glycosylation.
Endotoxin : < 1.0 EU per μg of the protein as determined by the LAL method
Purity : > 97 % as determined by SDS-PAGE
Storage : Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution : It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.
Gene Name : Sost sclerostin [ Rattus norvegicus ]
Official Symbol : Sost
Synonyms : SOST; sclerostin; sclerosteosis;
Gene ID : 80722
mRNA Refseq : NM_030584
Protein Refseq : NP_085073

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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