Active Recombinant Human Aminoacyl TRNA Synthetase Complex-Interacting Multifunctional Protein 1

• Cat.No.: AIMP1-338H
• Product Overview: Recombinant human AIMP1 protein was expressed in E. coli and purified by using conventional chromatography techniques.

Specification

• Cat. No.: AIMP1-338H
• Description: EMAP-II is a tumor derived cytokine that exerts a wide range of activities on endothelial cells, monocytes and neutrophils. EMAP-II inhibits endothelial cell proliferation, vasculogenesis, neovessel formation, and can induce apoptosis. It is also chemotactic towards neutrophils and monocytes and induces myeloperoxidase activity from neutrophils. Of clinical importance, EMAP-II inhibits angiogenesis of vascular beds and suppresses the growth of primary and secondary tumors without affecting normal tissues. Mature EMAP-II is an 18.3 kDa protein, which is synthesized as the C-terminal portion of a biologically inactive precursor protein containing a propeptide of 146 amino acid residues.
• Source: Human
• Host: E.coli
• Form: Supplied as a lyophilized powder.
• Purity: >98% pure by SDS PAGE and HPLC
• Sequence: Region of EMAP II protein corresponding to amino acids SKPIDVSRLD LRIGCIITAR KHPDADSLYV EEVDVGEIAP RTVVSGLVNH VPLEQMQNRM VILLCNLKPA KMRGVLSQAM VMCASSPEKI EILAPPNGSV PGDRITFDAF PGEPDKELNP KKKIWEQIQP DLHTNDECVA TYKGVPFEVK GKGVCRAQTM SNSGIK.
• Molecular Mass: 18.3 kDa
• Applications: Numerous applications are possible with this product and should be tested by the end user under their own laboratory conditions
• Endotoxin Levels: Endotoxin level is less than 0.1 ng per ug (1 EU/ug).
• Bioactivity: Determined by the apoptotic effect on MCF-7 cells using a concentration of 20-40 ng/ml.
• Storage: Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.
• Tag: Non

Gene Information

• Gene Name: AIMP1 aminoacyl tRNA synthetase complex-interacting multifunctional protein 1 [ Homo sapiens ]
• Official Symbol: AIMP1
• Synonyms: AIMP1; SCYE1; EMAP II; EMAP-2; EMAP-II; EMAP2; EMAPII; p43; small inducible cytokine subfamily E, member 1 (endothelial monocyte-activating); small inducible cytokine subfamily E, member 1; OTTHUMP00000161878; ARS-interacting multifunctional protein 1; endothelial monocyte-activating polypeptide 2; endothelial-monocyte activating polypeptide II; multisynthetase complex auxiliary component p43
• Gene ID: 9255
• mRNA Refseq: NM_001142415
• Protein Refseq: NP_001135887
• MIM: 603605
• UniProt ID: Q12904
• Chromosome Location: 4q2
• Pathway: Cytosolic tRNA aminoacylation; Gene Expression; tRNA Aminoacylation
• Function: cell surface binding; cytokine activity; protein binding; protein homodimerization activity; tRNA binding.

Reviews

09/14/2021

heir dedicated team of experts is readily available to address any challenges or questions that may arise during my experiments.

06/19/2016

Their profound knowledge and guidance provide invaluable assistance in overcoming hurdles and optimizing my research protocols, ensuring a seamless and efficient workflow.

03/12/2016

Its exceptional purity, stability, and functionality make it a reliable tool for conducting precise and reliable research, opening up new avenues for exploration and discovery.

04/16/2015

What truly sets this protein apart is the manufacturer's unwavering commitment to delivering exceptional technical support.

Q&As

How does AIMP1 affect cellular metabolism?

AIMP1 has been shown to affect cellular metabolism by regulating the activity of several metabolic enzymes. One of the known mechanisms of AIMP1-mediated metabolic regulation involves the inhibition of AMP-activated protein kinase (AMPK), a key regulator of cellular metabolism that responds to changes in energy balance. AIMP1 binds to and inhibits AMPK, leading to altered glucose and lipid metabolism in cells.

Can AIMP1 be used as a diagnostic or prognostic marker for disease?

AIMP1 has potential as a diagnostic or prognostic marker for certain diseases, particularly cancer. Its expression has been shown to be increased in several types of cancer, and high levels of AIMP1 expression have been associated with poor prognosis in some cancers. Additionally, AIMP1 expression has been suggested as a biomarker for early detection and progression monitoring of certain cancers.

Can AIMP1 protein be used as a therapeutic target?

AIMP1 has been proposed as a potential therapeutic target for various diseases, including cancer and neurological disorders. However, more research is needed to fully understand the therapeutic potential of targeting AIMP1.

What diseases are associated with mutations in the AIMP1 gene?

Mutations in the AIMP1 gene have been associated with various diseases, including rare neurological disorders such as leukodystrophies and movement disorders. Mutations in this gene have also been linked to susceptibility to certain types of cancers such as colorectal cancer and gastric cancer.

How is AIMP1 involved in neurological disorders?

Mutations in AIMP1 have been linked to several neurological disorders, including Charcot-Marie-Tooth disease type 2D, hereditary sensory and autonomic neuropathy type 2, and peripheral neuropathy with or without agenesis of the corpus callosum. AIMP1 is thought to play a role in the maintenance of peripheral nerves, and mutations in this gene can lead to peripheral nerve degeneration.

How is AIMP1 regulated in the cell?

AIMP1 protein expression and function are regulated by various mechanisms, including transcriptional regulation, post-transcriptional regulation, and post-translational modifications. For example, AIMP1 expression can be regulated by various transcription factors, such as PPARγ, NF-κB, and YY1. In addition, AIMP1 protein stability and activity can be regulated by phosphorylation, acetylation, and ubiquitination.

Can AIMP1 be used as a therapeutic target in autoimmune diseases?

AIMP1 has been suggested as a potential therapeutic target in autoimmune diseases such as rheumatoid arthritis. Inhibition of AIMP1 has been shown to reduce the severity of arthritis in mouse models, possibly by reducing inflammation and modulating immune cell differentiation. However, more research is needed to determine the efficacy and safety of targeting AIMP1 for therapeutic purposes in humans.

How is AIMP1 regulated at the transcriptional level?

The regulation of AIMP1 expression is complex and not yet fully understood. Multiple transcription factors, including NF-κB, p53, and c-Myc, have been shown to regulate AIMP1 expression by binding to its promoter region. In addition, epigenetic modifications, such as DNA methylation and histone acetylation, can also affect AIMP1 expression. Other factors, such as microRNA, can also regulate AIMP1 expression at the post-transcriptional level.

Are there any drugs targeting AIMP1 protein currently in development?

There are currently no drugs specifically targeting AIMP1 protein in development. But given the growing interest in AIMP1 as a therapeutic target, it is possible that such drugs may be developed in the future.

What techniques are used to study AIMP1 protein?

Various techniques can be used to study AIMP1 protein, including Western blot, immunofluorescence, co-immunoprecipitation, and mass spectrometry. These techniques can be used to study AIMP1 protein expression, localization, and protein-protein interactions.

Are there any drugs that target AIMP1?

Currently, there are no drugs that specifically target AIMP1. However, AIMP1 has been suggested as a potential therapeutic target for certain diseases, including cancer, and there is ongoing research to develop drugs that can modulate the activity of AIMP1. For example, small molecule inhibitors of AIMP1 have been identified and shown to have potential as cancer therapeutics in preclinical studies.