| Description : |
EMAP-II is a tumor derived cytokine that exerts a wide range of activities on endothelial cells, monocytes and neutrophils. EMAP-II inhibits endothelial cell proliferation, vasculogenesis, neovessel formation, and can induce apoptosis. It is also chemotactic towards neutrophils and monocytes and induces myeloperoxidase activity from neutrophils. Of clinical importance, EMAP-II inhibits angiogenesis of vascular beds and suppresses the growth of primary and secondary tumors without affecting normal tissues. Mature EMAP-II is an 18.3 kDa protein, which is synthesized as the C-terminal portion of a biologically inactive precursor protein containing a propeptide of 146 amino acid residues. |
| Source : |
E. coli |
| Species : |
Human |
| Form : |
Sterile Filtered White lyophilized (freeze-dried) powder. |
| Bio-activity : |
Determined by the apoptotic effect on MCF-7 cells using a concentration of 20-40 ng/mL, corresponding to a Specific Activity of ＞2.5 × 10^4 IU/mg. |
| Molecular Mass : |
Approximately 18.3 KDa, a single non-glycosylated polypeptide chain containing 166 amino acids. |
| Protein Length : |
166 |
| AA Sequence : |
SKPIDVSRLDLRIGCIITARKHPDA DSLYVEEVDVGEIAPRTVVSGLVNH VPLEQMQNRMVILLCNLKPAKMRGV LSQAMVMCASSPEKIEILAPPNGSV PGDRITFDAFPGEPDKELNPKKKIW EQIQPDLHTNDECVATYKGVPFEVK GKGVCRAQTMSNSGIK |
| Endotoxin : |
Less than 1 EU/mg of rHuEMAP-II as determined by LAL method. |
| Purity : |
>98% by SDS-PAGE and HPLC analyses. |
| Storage : |
This lyophilized preparation is stable at 2-8 centigrade, but should be kept at -20 centigrade for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 centigrade. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 to -70 centigrade. Avoid repeated freeze/thaw cycles. |
| Storage Buffer : |
Lyophilized from a 0.2mm filtered concentrated solution in 20mM PB, pH 7.4, 130mM NaCl. |
| Reconstitution : |
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at < -20 centigrade. Further dilutions should be made in appropriate buffered solutions. |
