Active Recombinant Human CD274 Protein, LIgG2b Fc-tagged, low endotoxin
Cat.No. : | CD274-379H |
Product Overview : | Recombinant Human CD274 Protein, LIgG2b Fc-tagged, low endotoxin is expressed from human 293 cells (HEK293). It contains AA Phe 19- Arg 238 (Accession # Q9NZQ7-1). Predicted N-terminus: Phe 19. This protein carries a llama IgG2b Fc tag at the C-terminus. The protein has a calculated MW of 53.0 kDa. The protein migrates as 65-95 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation. |
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Source : | HEK293 |
Species : | Human |
Tag : | lIgG2b |
Form : | Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, pH7.5. Normally trehalose is added as protectant before lyophilization. |
Bio-activity : | Immobilized Human PD-1, His Tag at 1 μg/mL (100 μL/well) can bind Recombinant Human CD274 Protein, LIgG2b Fc-tagged, low endotoxin with a linear range of 0.078-2.5 μg/mL (QC tested). Immobilized Recombinant Human CD274 Protein, LIgG2b Fc-tagged, low endotoxin at 2 μg/mL (100 μL/well) can bind Biotinylated Human PD-1, Fc Tag with a linear range of 20-313 ng/mL (Routinely tested). |
Molecular Mass : | 53.0 kDa |
Protein length : | Phe 19- Arg 238 |
Endotoxin : | Less than 0.01 EU per μg by the LAL method. |
Purity : | >95% as determined by SDS-PAGE. |
Notes : | Please avoid repeated freeze-thaw cycles. |
Stability : | No activity loss is observed after storage at: 4-8 centigrade for 12 months in lyophilized state; -70 centigrade for 3 months under sterile conditions after reconstitution. |
Storage : | For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower. |
Reconstitution : | It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 mg/ml. Centrifuge the vial at 4 centigrade before opening to recover the entire contents. |
Gene Name : | CD274 CD274 molecule [ Homo sapiens ] |
Official Symbol : | CD274 |
Synonyms : | CD274; CD274 molecule; B7 homolog 1; B7 H; B7 H1; B7H1; PD L1; PDL1; CD274 antigen; PDCD1 ligand 1; B7-H; PD-L1; PDCD1L1; PDCD1LG1; MGC142294; MGC142296; |
Gene ID : | 29126 |
mRNA Refseq : | NM_014143 |
Protein Refseq : | NP_054862 |
MIM : | 605402 |
UniProt ID : | Q9NZQ7 |
Products Types
◆ Recombinant Protein | ||
CD274-199C | Recombinant Human CD274 Protein, His-tagged | +Inquiry |
CD274-709H | Recombinant Human CD274 Protein, Fc-tagged | +Inquiry |
CD274-558R | Recombinant Rhesus Macaque CD274 Protein, His (Fc)-Avi-tagged | +Inquiry |
Cd274-25M | Recombinant Mouse CD274 Protein, Fc-His-tagged(C-ter) | +Inquiry |
CD274-713M | Recombinant Mouse CD274 Protein, Fc-tagged | +Inquiry |
◆ Lysates | ||
CD274-914CCL | Recombinant Cynomolgus CD274 cell lysate | +Inquiry |
CD274-2642MCL | Recombinant Mouse CD274 cell lysate | +Inquiry |
CD274-002RCL | Recombinant Rat CD274 cell lysate | +Inquiry |
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For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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