Active Recombinant Mouse Il3 Protein (135 aa)

• Cat.No.: Il3-027I
• Product Overview: Recombinant Mouse Il3 Protein without tag was expressed in E. coli.

Specification

• Description: IL-3 is a hematopoietic growth factor that promotes the survival, differentiation and proliferation of committed progenitor cells of the megakaryocyte, granulocyte-macrophage, erythroid, eosinophil, basophil and mast cell lineages. Produced by T cells, mast cells and eosinophils, IL-3 enhances thrombopoieses, phagocytosis, and antibody-mediated cellular cytotoxicity. Its ability to activate monocytes suggests that IL-3 may have additional immunoregulatory roles. Many of the IL-3 activities depend upon co-stimulation with other cytokines. IL-3 is species-specific, variably glycosylated cytokine.
• Source: E. coli
• Species: Mouse
• Form: Sterile Filtered White lyophilized (freeze-dried) powder.
• Bio-activity: The ED50 as determined by the dose-dependent stimulation of the proliferation of murine M-NFS-60 cells is < 0.05 ng/mL, corresponding to a specific activity of > 2 × 10^7 units/mg.
• Molecular Mass: Approximately 15.1 kDa globular protein containing 135 amino acid residues.
• Protein length: 135
• AA Sequence: MDTHRLTRTLNCSSIVKEIIGKLPE PELKTDDEGPSLRNKSFRRVNLSKF VESQGEVDPEDRYVIKSNLQKLNCC LPTSANDSALPGVFIRDLDDFRKKL RFYMVHLNDLETVLTSRPPQPASGS VSPNRGTVEC
• Endotoxin: Less than 1 EU/mg of rmIL-3 as determined by LAL method.
• Purity: >98% by SDS-PAGE and HPLC analyses.
• Storage: This lyophilized preparation is stable at 2-8 centigrade, but should be kept at -20 centigrade for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 centigrade. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 to -70 centigrade. Avoid repeated freeze/thaw cycles.
• Storage Buffer: Lyophilized from a 0.2mm filtered solution in PBS, pH 7.4.
• Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at < -20 centigrade. Further dilutions should be made in appropriate buffered solutions.

Gene Information

• Gene Name: Il3 interleukin 3 [ Mus musculus ]
• Official Symbol: Il3
• Synonyms: IL3; interleukin 3; interleukin-3; mast cell growth factor; P-cell-stimulating factor; hematopoietic growth factor; multipotential colony-stimulating factor; BPA; PSF; HCGF; Il-3; MCGF; Csfmu
• Gene ID: 16187
• mRNA Refseq: NM_010556
• Protein Refseq: NP_034686
• UniProt ID: P01586

Reviews

06/20/2022

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03/08/2021

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12/19/2020

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Q&As

What is the difference between IL-3 and GM-CSF In vitro?

In vitro, IL-3 can induce differentiation and proliferation of myeloid lineage cells and is distinguished from GM-CSF by the relative increase in production of platelets, basophils, and mast cells.

What is the difference in clinical presentation of IL-3 compared to GM-CSF?

Although effective, IL-3 appears to present no therapeutic advantages compared to G-CSF and GM-CSF, and in direct comparison studies, IL-3 led to a considerably higher rate of adverse events than either of the other cytokines.

Since the structure of IL-3 is different between animals and humans, does it mean that il-3 is not necessary to maintain stable hematopoietic function?

mice deficient in both bc and IL-3 show the same phenotype as the bc-deficient mice, conclusively demonstrating that, for the most part, the GM-CSF/IL3/IL5 family is not essential for steady-state production of myeloid cells

How does the structure of IL-3 in animals differ from that in humans?

Unlike in humans, murine IL-3 has a second b chain receptor, bcIL3, which bears 91% homology to the bc chain.

Does loss of cytokine IL-3 affect normal granulocyte, monocyte, and basophil levels?

Knockout mice were also reported with surprisingly limited phenotypes. Despite the ability of the three cytokines to stimulate hematopoiesis, mice functionally deficient in all three cytokinesdisplay normal circulating levels of granulocytes, monocytes, and basophils.

IL-3 specific receptor subunit CD123 is overexpressed in many hematological malignancies,Is it overexpressed in normal tissues where inflammation occurs?

non-hematopoietic (e.g., epithelial cells, and fibroblasts) cell subsets can also produce il-3.

When is IL-3 activated by T cells?

Such as allergic inflammation and parasitic infestation.

Since basophils are the target and source of IL-3, can they regulate the secretion of IL-3 to treat allergic diseases?

IL-3 can regulate the survival of basophil granulocytes and cytokine production through autocrine, so as to achieve the purpose of treating allergic diseases.

T cell IL-3 expression is specifically regulated by which transcription factor?

Bhlhe40

Which hematopoietic and non-hematopoietic cell subsets express IL-3?

E.g., lymphocytes,epithelial cells, and fibroblasts

Are there any completed clinical trials on IL3?

Yes, there are some completed clinical trials on IL3 results, but the specific results need to refer to the relevant research papers.

What is the effect of IL3 on hematopoietic system?

This protein can promote stem cell proliferation and differentiation during hematopoietic process, thus increasing the production of different types of blood cells.

Are there any side effects of IL3?

In general, the IL3 has relatively few side effects, but some unpleasant symptoms such as fever, headache and nausea may occur.

IL3 associated with whether the immune adjustment?

IL3 can regulate the body's immune response and the function of immune cells, and plays an important role in immune regulation.

What is the solubility and stability of IL3?

IL3 generally have good solubility and stability, making them easier to handle during drug preparation and storage.

How is the research progress of IL3?

The study of IL3 is ongoing, including its application in the development of therapeutic drugs, as well as in combination therapy with other immune molecules.