Recombinant Human ACOT8 293 Cell Lysate

• Cat.No.: ACOT8-9086HCL

Specification

• Description: Antigen standard for acyl-CoA thioesterase 8 (ACOT8), transcript variant 1 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
• Source: HEK 293 cells
• Species: Human
• Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
• Size: 0.1 mg
• Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
• Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.

Gene Information

• Gene Name: ACOT8 acyl-CoA thioesterase 8 [ Homo sapiens ]
• Official Symbol: ACOT8
• Synonyms: ACOT8; acyl-CoA thioesterase 8; peroxisomal acyl CoA thioesterase , peroxisomal acyl CoA thioesterase 1 , PTE1; acyl-coenzyme A thioesterase 8; choloyl CoA hydrolase; hACTE III; hTE; PTE 2; thioesterase II; thioesterase III; choloyl-CoA hydrolase; palmitoyl-CoA hydrolase; choloyl-coenzyme A thioesterase; long-chain fatty-acyl-CoA hydrolase; peroxisomal acyl-CoA thioesterase 1; HIV-Nef associated acyl-CoA thioesterase; peroxisomal long-chain acyl-CoA thioesterase 1; peroxisomal acyl-coenzyme A thioester hydrolase 1; PTE1; PTE2; PTE-1; PTE-2; HNAACTE; hACTE-III
• Gene ID: 10005
• mRNA Refseq: NM_005469
• Protein Refseq: NP_005460
• MIM: 608123
• UniProt ID: O14734
• Chromosome Location: 20pter-p12.3
• Pathway: Beta-oxidation of pristanoyl-CoA, organism-specific biosystem; Beta-oxidation of very long chain fatty acids, organism-specific biosystem; Bile acid and bile salt metabolism, organism-specific biosystem; Bile acid biosynthesis, cholesterol =>cholate, organism-specific biosystem; Bile acid biosynthesis, cholesterol => cholate, conserved biosystem
• Function: acyl-CoA hydrolase activity; carboxylesterase activity; choloyl-CoA hydrolase activity; hydrolase activity; protein binding

Q&As

What is the physiological relevance of ACOT7 in different tissues?

ACOT7 is expressed in a variety of tissues, including adipose tissue, liver, and skeletal muscle, where it plays important roles in regulating lipid metabolism and energy production. In adipose tissue, ACOT7 helps to control adiposity and improve glucose tolerance, while in liver it helps to reduce the accumulation of toxic lipids that are associated with NAFLD. In skeletal muscle, ACOT7 may help to regulate the production of ATP and the breakdown of glycogen, which are important for exercise performance.

Can ACOT7 protein be used as a drug target for treating metabolic disorders?

Yes, ACOT7 protein has been proposed as a drug target for treating metabolic disorders such as obesity, insulin resistance, and non-alcoholic fatty liver disease. However, more research is needed to fully understand the mechanisms of action and develop safe and effective ACOT7-targeted therapies.

How is ACOT7 regulated in cells?

The regulation of ACOT7 in cells is complex and not completely understood. Several studies have suggested that ACOT7 expression and activity may be regulated by factors such as fatty acid availability, insulin signaling, and post-translational modifications such as phosphorylation and acetylation. However, the precise mechanisms by which these factors regulate ACOT7 are still being investigated.

What experimental tools are used to study ACOT7 protein?

Several experimental techniques are used to study ACOT7, including genetic manipulation in cells and animal models, biochemical assays, and structural studies. For example, genetic manipulation techniques such as RNA interference and CRISPR/Cas9 can be used to study the effects of ACOT7 knockdown or overexpression in cells. Biochemical assays such as thin-layer chromatography and mass spectrometry are used to measure the activity of ACOT7 and its substrate specificity. Structural studies such as X-ray crystallography and nuclear magnetic resonance spectroscopy are used to determine the 3D structure of ACOT7 and its interaction with substrates and inhibitors.

What are the potential clinical applications of ACOT7 protein in medicine?

ACOT7 protein has potential clinical applications in the treatment of metabolic disorders such as obesity, insulin resistance, and non-alcoholic fatty liver disease. It may also have a potential role in improving exercise endurance and muscle health.

Can ACOT7 be targeted for drug development?

The dysregulation of ACOT7 has been implicated in several metabolic disorders, suggesting that it could be a potential target for drug development. However, several challenges must be overcome in developing ACOT7-targeted therapies, including the need to develop inhibitors that are selective for ACOT7 and do not inhibit other acyl-CoA thioesterases with different functions. Furthermore, given the crucial role of acyl-CoA esters in lipid metabolism and energy production, careful consideration must be given to potential side effects of ACOT7 inhibition.

How can ACOT7 protein be used to improve exercise performance?

ACOT7 protein may play a role in regulating energy production and glucose metabolism in skeletal muscle, which can affect exercise performance. Overexpression of ACOT7 in skeletal muscle has been shown to improve exercise endurance and increase glucose uptake in animal models. However, more research is needed to fully understand the role of ACOT7 in exercise physiology and its potential use in improving exercise performance.

What diseases are associated with dysregulation of ACOT7?

Dysregulation of ACOT7 has been implicated in several metabolic disorders, including obesity, insulin resistance, and non-alcoholic fatty liver disease (NAFLD). Studies in animal models have shown that overexpression of ACOT7 in adipose tissue can lead to improved glucose tolerance and reduced adiposity, suggesting a potential therapeutic role for ACOT7 in metabolic disorders.

How is ACOT7 involved in lipid metabolism?

ACOT7 plays an important role in lipid metabolism by catalyzing the hydrolysis of acyl-CoA esters, which are important intermediates in lipid metabolism. By breaking down acyl-CoA esters, ACOT7 helps regulate the levels of these intermediates and prevent excessive accumulation of lipids that can lead to metabolic disorders such as NAFLD. Additionally, studies have suggested that ACOT7 may play a role in regulating lipid oxidation and energy production in skeletal muscle.

Are there any natural compounds that target ACOT7?

Several natural compounds have been identified that can inhibit ACOT7 activity, including flavonoids such as quercetin and orientin, and polyphenols such as resveratrol and curcumin. However, the specificity and potency of these compounds and their potential therapeutic use in metabolic disorders requires further study.

What are some potential techniques for delivering ACOT7 protein to target tissues?

One potential technique for delivering ACOT7 protein to target tissues is through the use of gene therapy, in which a gene encoding for ACOT7 is introduced into target cells using viral vectors. Another potential approach is the use of cell-based therapies using stem cells or other cell types that overexpress ACOT7. However, both of these approaches require further research to assess their safety and efficacy.

What are the challenges in targeting ACOT7 for therapeutic purposes?

One of the main challenges in targeting ACOT7 for therapeutic purposes is to achieve specific inhibition of ACOT7 without affecting other proteins that share similar functions in lipid metabolism. Additionally, there may be potential side effects associated with modulating ACOT7 activity, and the long-term safety and efficacy of ACOT7-targeted therapies needs to be carefully evaluated.

What is the role of ACOT7 in exercise physiology?

The role of ACOT7 in exercise physiology is not fully understood, but studies have suggested that it may play a role in regulating energy production and glucose metabolism in skeletal muscle. In animal models, overexpression of ACOT7 in skeletal muscle has been shown to improve exercise endurance and increase glucose uptake. Additionally, levels of ACOT7 in skeletal muscle have been shown to increase in response to exercise, suggesting a potential role in adaptation to physical activity.