Recombinant Full Length Human ACOT8 Protein, GST-tagged
Cat.No. : | ACOT8-793HF |
Product Overview : | Human ACOT8 full-length ORF ( NP_005460.2, 1 a.a. - 319 a.a.) recombinant protein with GST-tag at N-terminal. |
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Description : | The protein encoded by this gene is a peroxisomal thioesterase that appears to be involved more in the oxidation of fatty acids rather than in their formation. The encoded protein can bind to the human immunodeficiency virus-1 protein Nef, and mediate Nef-induced down-regulation of CD4 in T-cells. [provided by RefSeq, Oct 2010] |
Source : | In Vitro Cell Free System |
Species : | Human |
Tag : | GST |
Molecular Mass : | 62.3 kDa |
Protein Length : | 319 amino acids |
AA Sequence : | MSSPQAPEDG QGCGDRGDPP GDLRSVLVTT VLNLEPLDED LFRGRHYWVP AKRLFGGQIV GQALVAAAKS VSEDVHVHSL HCYFVRAGDP KLPVLYQVER TRTGSSFSVR SVKAVQHGKP IFICQASFQQ AQPSPMQHQF SMPTVPPPEE LLDCETLIDQ YLRDPNLQKR YPLALNRIAA QEVPIEIKPV NPSPLSQLQR MEPKQMFWVR ARGYIGEGDM KMHCCVAAYI SDYAFLGTAL LPHQWQHKVH FMVSLDHSMW FHAPFRADHW MLYECESPWA GGSRGLVHGR LWRQDGVLAV TCAQEGVIRV KPQVSESKL |
Applications : | Enzyme-linked Immunoabsorbent Assay Western Blot (Recombinant protein) Antibody Production Protein Array |
Storage : | Store at -80 centigrade. Aliquot to avoid repeated freezing and thawing. |
Storage Buffer : | 50 mM Tris-HCl, 10 mM reduced Glutathione, pH=8.0 in the elution buffer. |
Gene Name : | ACOT8 acyl-CoA thioesterase 8 [ Homo sapiens ] |
Official Symbol : | ACOT8 |
Synonyms : | ACOT8; acyl-CoA thioesterase 8; peroxisomal acyl CoA thioesterase , peroxisomal acyl CoA thioesterase 1 , PTE1; acyl-coenzyme A thioesterase 8; choloyl CoA hydrolase; hACTE III; hTE; PTE 2; thioesterase II; thioesterase III; choloyl-CoA hydrolase; palmitoyl-CoA hydrolase; choloyl-coenzyme A thioesterase; long-chain fatty-acyl-CoA hydrolase; peroxisomal acyl-CoA thioesterase 1; HIV-Nef associated acyl-CoA thioesterase; peroxisomal long-chain acyl-CoA thioesterase 1; peroxisomal acyl-coenzyme A thioester hydrolase 1; PTE1; PTE2; PTE-1; PTE-2; HNAACTE; hACTE-III |
Gene ID : | 10005 |
mRNA Refseq : | NM_005469 |
Protein Refseq : | NP_005460 |
MIM : | 608123 |
UniProt ID : | O14734 |
Products Types
◆ Recombinant Protein | ||
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ACOT8-906H | Recombinant Human ACOT8 Protein, His-tagged | +Inquiry |
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ACOT8-3141H | Recombinant Human ACOT8, His-tagged | +Inquiry |
◆ Lysates | ||
ACOT8-9086HCL | Recombinant Human ACOT8 293 Cell Lysate | +Inquiry |
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For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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Q&As (13)
Ask a questionACOT7 is expressed in a variety of tissues, including adipose tissue, liver, and skeletal muscle, where it plays important roles in regulating lipid metabolism and energy production. In adipose tissue, ACOT7 helps to control adiposity and improve glucose tolerance, while in liver it helps to reduce the accumulation of toxic lipids that are associated with NAFLD. In skeletal muscle, ACOT7 may help to regulate the production of ATP and the breakdown of glycogen, which are important for exercise performance.
Yes, ACOT7 protein has been proposed as a drug target for treating metabolic disorders such as obesity, insulin resistance, and non-alcoholic fatty liver disease. However, more research is needed to fully understand the mechanisms of action and develop safe and effective ACOT7-targeted therapies.
The regulation of ACOT7 in cells is complex and not completely understood. Several studies have suggested that ACOT7 expression and activity may be regulated by factors such as fatty acid availability, insulin signaling, and post-translational modifications such as phosphorylation and acetylation. However, the precise mechanisms by which these factors regulate ACOT7 are still being investigated.
Several experimental techniques are used to study ACOT7, including genetic manipulation in cells and animal models, biochemical assays, and structural studies. For example, genetic manipulation techniques such as RNA interference and CRISPR/Cas9 can be used to study the effects of ACOT7 knockdown or overexpression in cells. Biochemical assays such as thin-layer chromatography and mass spectrometry are used to measure the activity of ACOT7 and its substrate specificity. Structural studies such as X-ray crystallography and nuclear magnetic resonance spectroscopy are used to determine the 3D structure of ACOT7 and its interaction with substrates and inhibitors.
ACOT7 protein has potential clinical applications in the treatment of metabolic disorders such as obesity, insulin resistance, and non-alcoholic fatty liver disease. It may also have a potential role in improving exercise endurance and muscle health.
The dysregulation of ACOT7 has been implicated in several metabolic disorders, suggesting that it could be a potential target for drug development. However, several challenges must be overcome in developing ACOT7-targeted therapies, including the need to develop inhibitors that are selective for ACOT7 and do not inhibit other acyl-CoA thioesterases with different functions. Furthermore, given the crucial role of acyl-CoA esters in lipid metabolism and energy production, careful consideration must be given to potential side effects of ACOT7 inhibition.
ACOT7 protein may play a role in regulating energy production and glucose metabolism in skeletal muscle, which can affect exercise performance. Overexpression of ACOT7 in skeletal muscle has been shown to improve exercise endurance and increase glucose uptake in animal models. However, more research is needed to fully understand the role of ACOT7 in exercise physiology and its potential use in improving exercise performance.
Dysregulation of ACOT7 has been implicated in several metabolic disorders, including obesity, insulin resistance, and non-alcoholic fatty liver disease (NAFLD). Studies in animal models have shown that overexpression of ACOT7 in adipose tissue can lead to improved glucose tolerance and reduced adiposity, suggesting a potential therapeutic role for ACOT7 in metabolic disorders.
ACOT7 plays an important role in lipid metabolism by catalyzing the hydrolysis of acyl-CoA esters, which are important intermediates in lipid metabolism. By breaking down acyl-CoA esters, ACOT7 helps regulate the levels of these intermediates and prevent excessive accumulation of lipids that can lead to metabolic disorders such as NAFLD. Additionally, studies have suggested that ACOT7 may play a role in regulating lipid oxidation and energy production in skeletal muscle.
Several natural compounds have been identified that can inhibit ACOT7 activity, including flavonoids such as quercetin and orientin, and polyphenols such as resveratrol and curcumin. However, the specificity and potency of these compounds and their potential therapeutic use in metabolic disorders requires further study.
One potential technique for delivering ACOT7 protein to target tissues is through the use of gene therapy, in which a gene encoding for ACOT7 is introduced into target cells using viral vectors. Another potential approach is the use of cell-based therapies using stem cells or other cell types that overexpress ACOT7. However, both of these approaches require further research to assess their safety and efficacy.
One of the main challenges in targeting ACOT7 for therapeutic purposes is to achieve specific inhibition of ACOT7 without affecting other proteins that share similar functions in lipid metabolism. Additionally, there may be potential side effects associated with modulating ACOT7 activity, and the long-term safety and efficacy of ACOT7-targeted therapies needs to be carefully evaluated.
The role of ACOT7 in exercise physiology is not fully understood, but studies have suggested that it may play a role in regulating energy production and glucose metabolism in skeletal muscle. In animal models, overexpression of ACOT7 in skeletal muscle has been shown to improve exercise endurance and increase glucose uptake. Additionally, levels of ACOT7 in skeletal muscle have been shown to increase in response to exercise, suggesting a potential role in adaptation to physical activity.
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