Recombinant Human MMP8 cell lysate

• Cat.No.: MMP8-2054HCL
• Product Overview: Human MMP-8 / CLG1 derived in Human Cells. The whole cell lysate is provided in 1X Sample Buffer.Browse all transfected cell lysate positive controls

Specification

• Source: Human cells
• Species: Human
• Preparation method: Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation and then centrifuged to clarify the lysate. The cell lysate was boiled for 5 minutes in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
• Lysis buffer: Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
• Quality control Testing: 12.5% SDS-PAGE Stained with Coomassie Blue
• Recommended Usage: 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200μL pure water and boiled for 2-5 min.3. Store it at -80°C. Recommend to aliquot the cell lysate into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.Notes:The lysate is ready to load on SDS-PAGE for Western blot application. If dissociating conditions are required, add reducing agent prior to heating.
• Stability: Samples are stable for up to twelve months from date of receipt at -80°C
• Storage Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
• Storage Instruction: Lysate samples are stable for 12 months from date of receipt when stored at -80°C. Avoid repeated freeze-thaw cycles. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes.
• Tag: Non

Gene Information

• Gene Name: MMP8 matrix metallopeptidase 8 (neutrophil collagenase) [ Homo sapiens ]
• Official Symbol: MMP8
• Synonyms: MMP8; matrix metallopeptidase 8 (neutrophil collagenase); CLG1, matrix metalloproteinase 8 (neutrophil collagenase); neutrophil collagenase; PMNL collagenase; matrix metalloproteinase-8; matrix metalloproteinase 8 (neutrophil collagenase); HNC; CLG1; MMP-8; PMNL-CL
• Gene ID: 4317
• mRNA Refseq: NM_002424
• Protein Refseq: NP_002415
• MIM: 120355
• UniProt ID: P22894
• Chromosome Location: 11q21-q22
• Pathway: Activation of Matrix Metalloproteinases, organism-specific biosystem; Degradation of the extracellular matrix, organism-specific biosystem; Extracellular matrix organization, organism-specific biosystem; Matrix Metalloproteinases, organism-specific biosystem
• Function: calcium ion binding; metalloendopeptidase activity; peptidase activity; serine-type endopeptidase activity; zinc ion binding

Reviews

04/03/2022

The use of MMP8 as a catalyst can significantly increase the rate and yield of chemical reactions with impressive high catalytic efficiency.

09/14/2021

MMP8 is really impressive, the results of every experiment are very consistent, you can see that its production process is very careful.

03/20/2021

MMP8 are inexpensive to prepare and cost-effective, making them ideal for our study.

Q&As

What signaling pathways does the MMP8 protein interact with?

The MMP8 protein interacts with a variety of signaling pathways, including TNF-α, TGF-β, Wnt, and NF-κB.

What drugs interact with the MMP8 protein?

A number of inhibitors and modulators of MMP8 protein have been developed for the regulation of MMP8 activity and the treatment of related diseases.

Why does MMP8 protein play a role in tissue repair?

The MMP8 protein is involved in the tissue repair and wound healing process, by degrading damaged tissue and supporting the formation of new tissue.

What is the mechanism of action of MMP8 protein in inflammatory response?

MMP8 protein is involved in regulating the production of inflammation-related molecules and cell infiltration, promoting the occurrence and maintenance of inflammatory response.

Does MMP8 have a role in post-traumatic recovery?

MMP8 protein is involved in the inflammatory and remodeling processes of post-traumatic wound healing, contributing to wound repair and tissue regeneration.

Does the MMP8 protein study have implications for new drug development?

MMP8 protein plays an important role in the occurrence and progression of diseases, and studying the regulatory mechanism of MMP8 protein is helpful for the development of new drugs, such as inhibitors targeting MMP8 protein may be applied to the treatment of related diseases