Recombinant Mouse Ang Protein, MYC/DDK-tagged

• Cat.No.: Ang-602M
• Product Overview: Purified recombinant protein of full-length mouse angiogenin, ribonuclease, RNase A family, 5 (Ang), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Description: This gene encodes a member of the pancreatic ribonuclease A superfamily and is a potent inducer of neovascularization. The encoded protein is a secreted multifunctional tRNA-specific ribonuclease that promotes angiogenesis in response to angiogenetic stimuli such as hypoxia, mediates stress-induced translational repression by cleaving cellular tRNAs, stimulates cell proliferation by mediating rRNA transcription in prostate cancer cells, and is involved in neurite pathfinding. This gene resides in a cluster of highly related genes. It shares dual promoters and 5' exons with the ribonuclease, RNase A family 4 gene. Two alternatively spliced variants, with different 5' exons but the same coding exon, have been identified. Multiple pseudogenes have been found for this gene.
• Source: HEK293T
• Species: Mouse
• Tag: Myc&DDK
• Molecular Mass: 16.2 kDa
• Purity: >80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Ang angiogenin, ribonuclease, RNase A family, 5 [ Mus musculus (house mouse) ]
• Official Symbol: Ang
• Synonyms: Ang; angiogenin, ribonuclease, RNase A family, 5; Ang1; Rnase5; Rnase5a; AI385586; angiogenin; RNase 5; angiogenin, ribonuclease A family, member 1; angiogenin-1; ribonuclease 5; EC 3.1.27.-
• Gene ID: 11727
• mRNA Refseq: NM_007447
• Protein Refseq: NP_031473
• UniProt ID: P21570

Reviews

09/27/2022

Whether it's troubleshooting experimental hurdles or optimizing protocols, their dedicated team is there to guide me, ensuring smooth progress and successful outcomes.

05/12/2022

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11/12/2020

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01/16/2019

the exemplary technical support provided by the manufacturer adds immense value to my experience.

04/01/2017

If needed, the availability of bulk purchase options further optimizes resource allocation, enabling me to conduct extensive experiments without compromising on quality.

Q&As

Can ANG protein be used as a therapeutic target?

Given its involvement in angiogenesis and disease processes, ANG protein has been explored as a therapeutic target. However, more research is needed to fully understand its complex biology and potential for targeted therapies.

Can ANG protein be used as a prognostic indicator for cancer?

ANG protein levels have been measured in various cancer types and have shown prognostic value in some cases. High levels of ANG protein have been associated with poor prognosis in certain cancers, indicating its potential as a prognostic biomarker.

What are the functions of ANG protein in neurodegenerative disorders?

In neurodegenerative disorders, ANG protein is thought to have multiple functions. It has been shown to promote the survival of neurons and protect them from various kinds of stress. ANG protein also possesses ribonucleolytic activity, where it can cleave RNA molecules, and this activity may be important for its neuroprotective effects.

Is ANG protein involved in other cellular processes besides angiogenesis?

Yes, ANG protein has been implicated in various cellular processes beyond angiogenesis. It has been found to play a role in cell survival, proliferation, migration, and RNA metabolism. ANG protein can also promote the growth of neurons and participate in synaptic plasticity.

Are there any known ANG protein mutations associated with diseases?

Yes, several mutations in the ANG gene have been identified in individuals with neurodegenerative disorders such as ALS. These mutations can impair ANG protein function, leading to the development of the disease.

Can ANG protein levels be measured in clinical settings to assess disease progression or treatment response?

ANG protein levels can be measured in clinical settings through various laboratory techniques, including enzyme-linked immunosorbent assays (ELISAs) or immunohistochemistry. However, the clinical utility of measuring ANG protein levels for assessing disease progression or treatment response remains to be fully established and is currently limited to specific research settings. Further studies are needed to determine its potential as a diagnostic or prognostic biomarker.

Is there a relationship between ANG protein and inflammation?

Yes, there is evidence to suggest a relationship between ANG protein and inflammation. Inflammatory signals can induce the expression of ANG protein, and elevated ANG protein levels have been detected in inflamed tissues. ANG protein has also been shown to interact with components of the immune system and modulate immune responses in certain contexts.

Where is ANG protein found?

ANG protein is found both intracellularly and extracellularly. It is present in various tissues, including endothelial cells, neurons, and certain cancer cells.

Are there any drugs or therapies targeting ANG protein in clinical trials?

There are currently no FDA-approved drugs specifically targeting ANG protein. However, some preclinical studies have explored the use of ANG inhibitors or activators in different disease models, including cancer. Clinical trials investigating the therapeutic potential of ANG protein-targeted therapies are limited but may emerge in the future.

Does ANG protein have any role in cardiovascular health?

ANG protein has been implicated in cardiovascular health and disease. It is involved in processes such as endothelial cell migration and vascular development. ANG protein has also been associated with conditions like atherosclerosis and ischemic heart disease, although further research is needed to fully understand its role in cardiovascular health and its potential as a therapeutic target.

Can ANG protein be used as a potential therapeutic target for neurodegenerative diseases?

ANG protein has been investigated as a potential therapeutic target for neurodegenerative diseases, including ALS. However, developing effective therapies targeting ANG protein is challenging due to its involvement in various cellular processes and the need to specifically modulate its functions without causing detrimental side effects. Further research is needed to better understand the role of ANG protein in disease pathogenesis and identify potential therapeutic strategies.

What are the medical implications of ANG protein?

ANG protein has been implicated in various diseases, including cancer, neurodegenerative disorders, and cardiovascular conditions. In cancer, ANG protein can promote tumor growth and metastasis by inducing angiogenesis. In neurodegenerative disorders, ANG protein dysfunction has been linked to diseases like ALS (amyotrophic lateral sclerosis).

Is ANG protein used in any diagnostic tests?

ANG protein levels have been measured in various disease states and can serve as a biomarker for certain conditions. For example, elevated ANG protein levels have been associated with certain cancers, making it potentially useful in diagnostic testing or monitoring disease progression.

Are there any known natural regulators or inhibitors of ANG protein activity?

Yes, there are natural regulators and inhibitors of ANG protein activity. One example is the ribonuclease inhibitor protein, which can bind to ANG protein and inhibit its ribonucleolytic activity. Additionally, certain microRNAs have been found to regulate ANG protein expression levels.

Are there any inhibitors or activators of ANG protein?

Yes, several small molecules and antibodies have been developed as inhibitors or activators of ANG protein. These molecules can be used for research purposes or potentially as therapeutic agents in treating diseases involving ANG protein dysregulation.

Does ANG protein play a role in the development of other neurological disorders besides ALS?

While ANG protein has been primarily studied in relation to ALS, there is evidence to suggest its potential involvement in other neurological disorders. For example, altered ANG protein levels and genetic variations in the ANG gene have been associated with conditions such as Alzheimer's disease, Parkinson's disease, and multiple sclerosis. However, more research is needed to fully elucidate the role of ANG protein in these disorders.

How is ANG protein related to amyotrophic lateral sclerosis (ALS)?

ANG protein has been linked to ALS, a neurodegenerative disorder, through genetic mutations. Certain mutations in the ANG gene can lead to a decrease in ANG protein production or impair its function. This can contribute to the development of ALS, as ANG protein is involved in various processes that are important for maintaining the health and function of motor neurons.

Can ANG protein be targeted for anti-angiogenic therapies?

ANG protein has been considered as a potential target for anti-angiogenic therapies. Inhibiting ANG protein's angiogenic activity could help in limiting pathological angiogenesis in diseases such as cancer. However, developing effective ANG inhibitors that specifically target pathological angiogenesis while sparing physiological angiogenesis remains a challenge.

Are there any animal models used to study ANG protein and its role in diseases?

Yes, various animal models have been used to study ANG protein and its role in diseases. For example, mice models with genetically modified ANG genes have been created to mimic certain mutations associated with neurodegenerative disorders like ALS. These models allow researchers to investigate the impact of ANG protein dysfunction on disease development and progression.

How is ANG protein regulated?

The expression and activity of ANG protein are regulated by various factors such as growth factors, cytokines, and cellular stress. Specific signaling pathways involving proteins like VEGF, HIF-1α, and PI3K/AKT are known to influence ANG protein regulation.