Recombinant Mouse Top1 Protein, His-tagged

• Cat.No.: Top1-465M
• Product Overview: Recombinant Mouse Top1 Protein (Met1-Ser252) with N-His tag was expressed in E. coli.

Specification

• Description: Enables DNA topoisomerase type I (single strand cut, ATP-independent) activity. Acts upstream of or within DNA replication; DNA topological change; and embryonic cleavage. Located in cytoplasm; nucleus; and perikaryon. Is expressed in brain; cerebral cortex ventricular layer; cortical plate; and telencephalon. Orthologous to human TOP1 (DNA topoisomerase I).
• Source: E. coli
• Species: Mouse
• Tag: His
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 34.1 kDa
  Accurate Molecular Mass: 34 kDa
• Protein length: Met1-Ser252
• Purity: > 97%
• Applications: Positive Control; Immunogen; SDS-PAGE; WB.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 centigrade for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 centigrade for one month. Aliquot and store at -80 centigrade for 12 months.
• Storage Buffer: 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
• Reconstitution: Reconstitute in sterile water to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Gene Information

• Gene Name: Top1 topoisomerase (DNA) I [ Mus musculus (house mouse) ]
• Official Symbol: Top1
• Synonyms: TOP1; topoisomerase (DNA) I; DNA topoisomerase 1; DNA topoisomerase I; Top-1; AI467334; D130064I21Rik
• Gene ID: 21969
• mRNA Refseq: NM_009408
• Protein Refseq: NP_033434

Reviews

10/17/2019

Accurate protein structural analysis; a key for structural biology.

10/06/2018

High-quality protein crystallography; essential for structural biology.

05/30/2018

Timely protein characterization; vital for our research projects.

Q&As

What is the role of TOP1 in the repair of DNA damage caused by environmental factors and chemotherapeutic agents?

TOP1 repairs DNA damage, maintaining genomic stability under stress.

How do post-translational modifications of TOP1, such as phosphorylation, affect its enzymatic activity and interaction with DNA?

Post-translational modifications like phosphorylation affect TOP1's activity and DNA interaction.

How do mutations in the TOP1 gene contribute to genomic instability and the development of certain cancers?

Mutations in TOP1 cause genomic instability, increasing cancer risk.

What are the effects of TOP1 inhibitors on cancer cell proliferation and survival?

TOP1 inhibitors lead to cancer cell death and inhibit tumor growth by disrupting DNA processes.

How does TOP1 interact with other proteins in the DNA repair and replication machinery?

TOP1 works with other DNA repair proteins, ensuring accurate DNA synthesis.

How does TOP1 regulate DNA unwinding and re-ligation during transcription and replication?

TOP1 regulates DNA unwinding by creating transient breaks, aiding transcription and replication.

What are the implications of TOP1 expression levels in predicting the response to chemotherapy in cancer patients?

TOP1 expression levels predict chemotherapy responses in cancer treatment.