Recombinant Human TOP1 protein(Lys191-Phe765), His-tagged

• Cat.No.: TOP1-1078H
• Product Overview: Recombinant Human TOP1 (NP_003277.1) (Lys191-Phe765) was expressed in Insect Cells with a polyhistidine tag at the N-terminus.

Specification

• Source: Insect Cells
• Species: Human
• Tag: N-His
• Protein length: Lys191-Phe765
• Form: Lyophilized from sterile 20 mM Tris, 200 mM NaCl, 20 % glycerol, pH 7.5. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Molecular Mass: The recombinant human TOP1 consists of 594 amino acids and predicts a molecular mass of 70.4 kDa.
• Endotoxin: < 1.0 EU per μg protein as determined by the LAL method.
• Purity: > 90 % as determined by SDS-PAGE.
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: TOP1 topoisomerase (DNA) I [ Homo sapiens ]
• Official Symbol: TOP1
• Synonyms: TOP1; topoisomerase (DNA) I; DNA topoisomerase 1; type I DNA topoisomerase; TOPI
• Gene ID: 7150
• mRNA Refseq: NM_003286
• Protein Refseq: NP_003277
• MIM: 126420
• UniProt ID: P11387