Recombinant Human ADCK2 293 Cell Lysate

• Cat.No.: ADCK2-9023HCL

Specification

• Description: Antigen standard for aarF domain containing kinase 2 (ADCK2) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
• Source: HEK 293 cells
• Species: Human
• Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
• Size: 0.1 mg
• Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
• Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.

Gene Information

• Gene Name: ADCK2 aarF domain containing kinase 2 [ Homo sapiens ]
• Official Symbol: ADCK2
• Synonyms: ADCK2; aarF domain containing kinase 2; uncharacterized aarF domain-containing protein kinase 2; MGC20727; putative ubiquinone biosynthesis protein AarF; AARF
• Gene ID: 90956
• mRNA Refseq: NM_052853
• Protein Refseq: NP_443085
• UniProt ID: Q7Z695
• Chromosome Location: 7q34
• Function: ATP binding; kinase activity; nucleotide binding; protein serine/threonine kinase activity; transferase activity

Q&As

What are the consequences of ADCK2 mutations?

Mutations in ADCK2 are associated with various diseases, including primary and secondary CoQ10 deficiencies, cerebellar ataxia, and steroid-resistant nephrotic syndrome. These mutations can affect the enzyme's ability to phosphorylate the intermediate compound in the CoQ10 biosynthesis pathway, leading to reduced levels of CoQ10 in tissues and cells. As CoQ10 plays important roles in energy production and protection against oxidative stress, CoQ10 deficiency can result in a range of symptoms, including muscle weakness, neurological problems, and kidney dysfunction.

Can ADCK2 protein be used for biomarker development?

Some studies have investigated ADCK2 protein levels as a potential biomarker for CoQ10 deficiency, but the results have been mixed and more research is needed to determine whether ADCK2 levels could be used for diagnostic or prognostic purposes. Additionally, ADCK2 mutations have been associated with several diseases, so testing for these mutations may be a useful diagnostic tool in certain populations.

Can ADCK2 protein be used to develop new drugs?

While there are currently no specific drugs targeting ADCK2 protein, the regulation of the CoQ10 biosynthesis pathway and its involvement in various diseases suggest that ADCK2 protein may be a useful target for drug development. Compounds that can increase CoQ10 biosynthesis or protect against oxidative stress may be potential therapeutic agents for diseases associated with CoQ10 deficiency.

Are there any risks associated with the use of ADCK2 protein therapeutically?

The use of ADCK2 protein as a therapeutic agent has not been extensively studied, so there may be potential risks and side effects that are not yet known.

How can ADCK2 protein be targeted for therapeutic interventions?

There is currently no specific drug targeting ADCK2 protein for therapeutic interventions. However, CoQ10 supplementation is widely used to treat CoQ10 deficiency-related disorders, and some studies have investigated compounds that can increase CoQ10 biosynthesis or protect against oxidative stress as potential therapeutic agents. Modulating the expression or activity of other proteins involved in the CoQ10 biosynthesis pathway may also be a potential strategy for increasing CoQ10 levels in cells or tissues.

What are the potential therapeutic applications of ADCK2 protein?

The potential therapeutic applications of ADCK2 protein are not well understood. However, ADCK2 protein has been linked to several metabolic pathways, including CoQ10 biosynthesis, suggesting that it may be a potential therapeutic target for conditions related to CoQ10 deficiency.

Can exercise affect ADCK2 protein levels?

There is limited research on the effects of exercise on ADCK2 protein levels, but some studies have suggested that exercise may help maintain mitochondrial function and reduce oxidative stress, which could potentially affect ADCK2 expression. However, more research is needed to confirm these findings.

Are there any dietary interventions that can affect ADCK2 protein levels?

The regulation of ADCK2 expression in the body is not yet fully understood, but some studies have suggested that dietary intake may play a role. For example, a diet high in fruits and vegetables, which are rich in antioxidants, may help protect against oxidative stress and maintain mitochondrial function, possibly affecting ADCK2 protein levels.

How is ADCK2 expression regulated in the body?

The regulation of ADCK2 expression in the body is not yet fully understood. Some studies have suggested that ADCK2 expression may be regulated by a transcription factor called Nrf2, which is involved in the cellular stress response and plays a role in maintaining mitochondrial function. Other studies have found that ADCK2 expression may be affected by a variety of other factors, such as dietary intake, aging, and exercise, but more research is needed to confirm these findings.

What are the potential applications of ADCK2 protein?

One potential application of ADCK2 protein is as a therapeutic target for the treatment of diseases associated with CoQ10 deficiency, such as primary and secondary CoQ10 deficiencies, cerebellar ataxia, and steroid-resistant nephrotic syndrome. In addition, ADCK2 protein levels or mutations may be useful biomarkers for diagnostic or prognostic purposes in certain populations.

Is there a diagnostic test for ADCK2 mutations?

Yes, diagnostic tests are available for ADCK2 mutations. These tests can be used to identify mutations associated with diseases such as cerebellar ataxia and steroid-resistant nephrotic syndrome.

Are there any clinical trials underway that focus on ADCK2 protein?

There are currently no clinical trials underway that focus on ADCK2 protein. However, there are ongoing studies investigating CoQ10 supplementation in various conditions, including heart disease and neurodegenerative disorders.

Can ADCK2 protein be used to diagnose CoQ10 deficiency?

ADCK2 protein levels may provide indirect information about CoQ10 levels in the body, but it is not a direct diagnostic tool for CoQ10 deficiency. Diagnosis of CoQ10 deficiency usually requires specific tests that measure CoQ10 levels in blood or tissues.