Recombinant Full Length Human Uncharacterized Aarf Domain-Containing Protein Kinase 2(Adck2) Protein, His-Tagged

• Cat.No.: RFL26479HF
• Product Overview: Recombinant Full Length Human Uncharacterized aarF domain-containing protein kinase 2(ADCK2) Protein (Q7Z695) (1-626aa), fused to N-terminal His tag, was expressed in E. coli.

Specification

• Source: E.coli expression system
• Species: Homo sapiens (Human)
• Tag: His
• Form: Lyophilized powder
• Protein Length: Full Length (1-626)
• AA Sequence: MVAPWRVSVRVCLSHLRCFELRQGL SLLRPSECPRDARLCWLLLGTLPKV VSLCGDVGEG APDVLSRRRVRCSGAAGAGPAESLP RAGPLGGVFLHLRLWLRAGALLVKF FPLLLLYPLT YLAPSVSTLWLHLLLKATETSGPTY IKLGQWASTRRDLFSEAFCAQFSKL HVRVTPHPWT HTERFLRQAFGDDWGSILSFENREP VGSGCVAQVYKAYANTAFLETDSVQ RLGRASCLPP FSHTGAVGGLRELFGYLGNGRKPPE NLADQSFLERLLLPKADLVGSNAGV SRAQVPGHQP EATNLISVAVKVLHPGLLAQVHMDL LLMKIGSRVLGVLPGIKWLSLPEIV EEFEKLMVQQ IDLRYEAQNLEHFQVNFRNVKAVKF PTPLRPFVTREVLVETYEESVPVSS YQQAGIPVDL KRKIARLGINMLLKMIFVDNFVHAD LHPGNILVQGANGLSSSQEAQLQQA DICDTLVVAV PSSLCPLRLVLLDAGIVAELQAPDL RNFRAVFMAVVMGQGQRVAELILHH ARASECRDVE GFKTEMAMLVTQARKNTITLEKLHV SSLLSSVFKLLMTHKVKLESNFASI VFAIMVLEGL GRSLDPKLDILEAARPFLLTGPVCP P
• Purity: Greater than 90% as determined by SDS-PAGE.
• Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
• Storage: Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
• Storage Buffer: Tris/PBS-based buffer, 6% Trehalose, pH 8.0
• Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
• Gene Name: ADCK2
• Synonyms: ADCK2; AARF; Uncharacterized aarF domain-containing protein kinase 2
• UniProt ID: Q7Z695

Gene Information

• Gene Name: ADCK2
• Synonyms: ADCK2; AARF; Uncharacterized aarF domain-containing protein kinase 2
• UniProt ID: Q7Z695

Q&As

What are the consequences of ADCK2 mutations?

Mutations in ADCK2 are associated with various diseases, including primary and secondary CoQ10 deficiencies, cerebellar ataxia, and steroid-resistant nephrotic syndrome. These mutations can affect the enzyme's ability to phosphorylate the intermediate compound in the CoQ10 biosynthesis pathway, leading to reduced levels of CoQ10 in tissues and cells. As CoQ10 plays important roles in energy production and protection against oxidative stress, CoQ10 deficiency can result in a range of symptoms, including muscle weakness, neurological problems, and kidney dysfunction.

Can ADCK2 protein be used for biomarker development?

Some studies have investigated ADCK2 protein levels as a potential biomarker for CoQ10 deficiency, but the results have been mixed and more research is needed to determine whether ADCK2 levels could be used for diagnostic or prognostic purposes. Additionally, ADCK2 mutations have been associated with several diseases, so testing for these mutations may be a useful diagnostic tool in certain populations.

Can ADCK2 protein be used to develop new drugs?

While there are currently no specific drugs targeting ADCK2 protein, the regulation of the CoQ10 biosynthesis pathway and its involvement in various diseases suggest that ADCK2 protein may be a useful target for drug development. Compounds that can increase CoQ10 biosynthesis or protect against oxidative stress may be potential therapeutic agents for diseases associated with CoQ10 deficiency.

Are there any risks associated with the use of ADCK2 protein therapeutically?

The use of ADCK2 protein as a therapeutic agent has not been extensively studied, so there may be potential risks and side effects that are not yet known.

How can ADCK2 protein be targeted for therapeutic interventions?

There is currently no specific drug targeting ADCK2 protein for therapeutic interventions. However, CoQ10 supplementation is widely used to treat CoQ10 deficiency-related disorders, and some studies have investigated compounds that can increase CoQ10 biosynthesis or protect against oxidative stress as potential therapeutic agents. Modulating the expression or activity of other proteins involved in the CoQ10 biosynthesis pathway may also be a potential strategy for increasing CoQ10 levels in cells or tissues.

What are the potential therapeutic applications of ADCK2 protein?

The potential therapeutic applications of ADCK2 protein are not well understood. However, ADCK2 protein has been linked to several metabolic pathways, including CoQ10 biosynthesis, suggesting that it may be a potential therapeutic target for conditions related to CoQ10 deficiency.

Can exercise affect ADCK2 protein levels?

There is limited research on the effects of exercise on ADCK2 protein levels, but some studies have suggested that exercise may help maintain mitochondrial function and reduce oxidative stress, which could potentially affect ADCK2 expression. However, more research is needed to confirm these findings.

Are there any dietary interventions that can affect ADCK2 protein levels?

The regulation of ADCK2 expression in the body is not yet fully understood, but some studies have suggested that dietary intake may play a role. For example, a diet high in fruits and vegetables, which are rich in antioxidants, may help protect against oxidative stress and maintain mitochondrial function, possibly affecting ADCK2 protein levels.

How is ADCK2 expression regulated in the body?

The regulation of ADCK2 expression in the body is not yet fully understood. Some studies have suggested that ADCK2 expression may be regulated by a transcription factor called Nrf2, which is involved in the cellular stress response and plays a role in maintaining mitochondrial function. Other studies have found that ADCK2 expression may be affected by a variety of other factors, such as dietary intake, aging, and exercise, but more research is needed to confirm these findings.

What are the potential applications of ADCK2 protein?

One potential application of ADCK2 protein is as a therapeutic target for the treatment of diseases associated with CoQ10 deficiency, such as primary and secondary CoQ10 deficiencies, cerebellar ataxia, and steroid-resistant nephrotic syndrome. In addition, ADCK2 protein levels or mutations may be useful biomarkers for diagnostic or prognostic purposes in certain populations.

Is there a diagnostic test for ADCK2 mutations?

Yes, diagnostic tests are available for ADCK2 mutations. These tests can be used to identify mutations associated with diseases such as cerebellar ataxia and steroid-resistant nephrotic syndrome.

Are there any clinical trials underway that focus on ADCK2 protein?

There are currently no clinical trials underway that focus on ADCK2 protein. However, there are ongoing studies investigating CoQ10 supplementation in various conditions, including heart disease and neurodegenerative disorders.

Can ADCK2 protein be used to diagnose CoQ10 deficiency?

ADCK2 protein levels may provide indirect information about CoQ10 levels in the body, but it is not a direct diagnostic tool for CoQ10 deficiency. Diagnosis of CoQ10 deficiency usually requires specific tests that measure CoQ10 levels in blood or tissues.