Address: 45-1 Ramsey Road, Shirley, NY 11967, USA    USA: 1-631-559-9269  1-631-448-7888   Fax: 1-631-938-8127  Europe: 44-207-048-3343   Email: info@creative-biomart.com
Home > Products > Assay Kits > Cellular Kits > 7-AAD/CFSE Cell-Mediated Cytotoxicity Assay Kit

7-AAD/CFSE Cell-Mediated Cytotoxicity Assay Kit

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Cat.No.:
Kit-1919
Size:
1 ea
Description:
The 7-AAD/CFSE Cell-Mediated Cytotoxicity Assay Kit employs CFSE to label target cells within the mixed cell population and 7-AAD to label dead cells. This kit provides an improvement over the traditional 51chromium (51Cr) release assay to assess cell-mediated cytotoxicity. CFSE labeling is more sensitive, does not employ radioisotopes, and cytolysis can be assessed at the single-cell level. The mixed lymphocyte reaction is a cornerstone method of immunology1, and this kit can help immunologists to detect subtle changes in cytotoxic lymphocyte function. The kit provides sufficient reagents to effectively stain approximately 10^9 cells.
Storage:
-20°C
Kit Components:
Cell-Based Assay 7-AAD Staining Stock Solution (1,000X): 2 vials/50 µl; 4°C CFSE Stock Solution: 1 vial/100 µl; -20°C Cell-Based Assay Buffer Tablet: 4 Tablets; RT


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Optional requirements on this protein +Expand
C-fusion   N-fusion  Non-tagged
His   GST  Fc  Others
<1.0 eu/μg   <0.1 eu/μg  <0.01 eu/μg  Not required
Monomer Isolation   Dimer Isolation   Not required
>80% by SDS-PAGE   >90% by SDS-PAGE  >95% by SDS-PAGE  Others
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45-1 Ramsey Road, Shirley, NY 11967, USA
USA: 1-631-559-9269  1-631-448-7888
Europe: 44-207-048-3343
FAX: 1-631-938-8127
EMAIL: info@creative-biomart.com

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