Address: 45-1 Ramsey Road, Shirley, NY 11967, USA    USA: 1-631-559-9269  1-631-448-7888   Fax: 1-631-938-8127  Europe: 44-207-048-3343   Email:
"AKT" Related Products.
Product Overview:
AKT Activity Assay Kit utilizes an Akt-specific antibody to immunoprecipitate Akt from cell lysate. Activity of the Akt is then determined in a kinase reaction using recombinant GSK-3a as substrate. Phosphorylation of the GSK-3a can be analyzed by Western blot analysis using the phospho-GSK-3a specific antibody included in the kit. The kit specifically detects Akt1, Akt2, and Akt3 activities, other kinase activities would not be detected. The AKT Specific Antibody reacts with human, mouse, and rat.
40 tests
Akt is a protein kinase that can be activated by insulin and various growth factors and functions in a pathway involving PI3 kinase. Recent evidence suggests that Akt functions to promote cell survival by actively inhibiting apoptosis.
Functional Studies more details
Store at -20°C.
Kit Components:
Components: Identifier; Akt Specific Antibody: Red; GSK-3a Protein/ATP Mixture: Blue; Kinase Assay Buffer: WM; Kinase Extraction Buffer: NM; Phospho-GSK-3a Specific Antibody: Green; Protein A Sepharose: Clear
Compatible Sample Types:
Tissue Extracts, Cell Lysate

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Note: There will be extra charge for optional service!
Optional requirements on this protein +Expand
C-fusion   N-fusion  Non-tagged
His   GST  Fc  Others
<1.0 eu/μg   <0.1 eu/μg  <0.01 eu/μg  Not required
Monomer Isolation   Dimer Isolation   Not required
>80% by SDS-PAGE   >90% by SDS-PAGE  >95% by SDS-PAGE  Others
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45-1 Ramsey Road, Shirley, NY 11967, USA
USA: 1-631-559-9269  1-631-448-7888
Europe: 44-207-048-3343
FAX: 1-631-938-8127

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