Address: 45-1 Ramsey Road, Shirley, NY 11967, USA    USA: 1-631-559-9269  1-631-448-7888   Fax: 1-631-938-8127  Europe: 44-207-048-3343   Email: info@creative-biomart.com
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Ammonia Fluorometric Assay Kit

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Cat.No.:
Kit-0086
Product Overview:
Ammonia Assay Kit is a quantitative fluorimetric determination of ammonia at FL360/450 nm.
Description:
AMMONIA (NH3) or its ion form ammonium (NH4+) is an important source of nitrogen for living systems and is ubiquitously present in the nature. Simple, direct and automation-ready procedures for measuring NH3 are very desirable. Ammonia Assay Kit is based on an improved o-phthalaldehyde method. This reagent reacts with ammonia/ ammonium and forms a fluorescent product. The fluorescence intensity (λex/em = 360/450nm) is proportional to the ammonia concentration in the sample.
Applications:
Ammonia/ammonium determination in biological (e.g. urine) and environmental samples.
Usage:
For research use only (RUO)
Storage:
Store kit at -20°C. Shelf life of 6 months after receipt.
Kit Components:
Assay Buffer 20 mLStandard (NH4Cl) 400 µLReagent A 1 mLReagent B 1 mL
Detection method:
Fluorimetric
Compatible Sample Types:
Environ-mental Sample, Urine
Features & Benefits:
Fast and sensitive. Linear detection range of 0.012 - 1 mM ammonia.Convenient and high-throughput. Homogeneous "mix-incubate-measure" type assay. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day.


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Optional requirements on this protein +Expand
C-fusion   N-fusion  Non-tagged
His   GST  Fc  Others
<1.0 eu/μg   <0.1 eu/μg  <0.01 eu/μg  Not required
Monomer Isolation   Dimer Isolation   Not required
>80% by SDS-PAGE   >90% by SDS-PAGE  >95% by SDS-PAGE  Others
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45-1 Ramsey Road, Shirley, NY 11967, USA
USA: 1-631-559-9269  1-631-448-7888
Europe: 44-207-048-3343
FAX: 1-631-938-8127
EMAIL: info@creative-biomart.com

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