Detection and Quantification of Aspartate Transaminase Activity.
Aspartate Transaminase (AST), also known as serum glutamic oxaloacetic transaminase (GOT) or aspartate aminotransferase (ASAT/AAT), facilitates the conversion of aspartate and a-ketoglutarate to oxaloacetate and glutamate. There are two isoenzymes in humans: GOT1 is a cytosolic isoenzyme derived from red blood cells and heart; GOT2 is the mitochondrial isoenzyme found mainly in the liver. AST is elevated in liver and muscle diseases. It is part of diagnostic tests for liver function, myocardial infarction, acute pancreatitis, acute hemolytic anemia, severe burns, acute renal disease and trauma.
Shipped and store at 4°C for 6 months.
Component-Volume-Storage 1. 96-Well Microplate; 2. Extract: 30 ml x 1, 4°C; 3. Reagent I: 5 ml x 1, 4°C; 4. Reagent II: 7.5 ml x 1, 4°C;5. Reagent III: 25 ml x 1, 4°C; 6. Technical Manual: 1 Manual
Compatible Sample Types:
Serum; Other biological fluids