"&beta" Related Products

Beta Secretase Activity Assay Kit

Cat.No.: Kit-0862
Product Overview: Beta Secretase Activity Assay Kit is a convenient fluorescence method for detecting beta secretase activity in biological and purified samples. The assay uses a secretase-specific peptide conjugated to two reporter molecules, EDANS and DABCYL. In the uncleaved form, the fluorescent signal from EDANS is quenched by the physical proximity of the DABCYL moiety. Cleavage of the peptide by beta secretase physically separates EDANS and DABCYL allowing for the release of a fluorescent signal. The level of secretase enzymatic activity in samples is proportional to the level of fluorescence intensity.
Size: 100 x 1 assay
Description: β-Secretase (Beta secretase) is responsible for the proteolytic process of the amyloid precursor protein (APP). It cleaves APP at the N-terminus of the A-beta peptide sequence, between residues 671-672, leading to the generation and extracellular release of beta-cleaved soluble APP, and a corresponding cell-associated C-terminal fragment which is later released by gamma-secretase. It has been implicated to be an excellent target for anti-amyloid therapy for the treatment of Alzheimer"s disease
Applications: Functional Studies
Target Species: Reacts with: Mouse, Rat, HumanPredicted to work with: all Mammals
Storage: Store kit at -20ºC in the dark immediately upon receipt. Kit has a storage time of 1 year from receipt, providing components have not been reconstituted.Refer to list of materials supplied for storage conditions of individual components. Observe the storage conditions for individual prepared components in section 5.Aliquot components in working volumes before storing at the recommended temperature. Reconstituted components are Stable for 6 months.
Kit Components: Components Identifier 100 testsActive ß -Secretase (Lyophilized) Red 1 vialß -Secretase Extraction Buffer NM 1 x 25mlß -Secretase Inhibitor (in DMSO) Blue 1 x 10µlß -Secretase Reaction Buffer (2X) WM 1 x 10mlß -Secretase Substrate (in DMSO) Amber NM 1 x 200µl
Compatible Sample Types: Urine, Serum, Plasma, Other biological fluids, Cell Lysate, Cell culture media, Tissue Lysate
Assay time: 1h 00m

Online Inquiry

Note: There will be extra charge for optional service!
Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

Please input "biomart" as verification code. Please review Creative BioMart's privacy policy for more information


Price does not include shipping and packaging costs.Request quote for final price.

Price Inquiry

Welcome! For price inquiries, please feel free to contact us through the form below. We will get back to you as soon as possible.