Address: 45-1 Ramsey Road, Shirley, NY 11967, USA    USA: 1-631-559-9269  1-631-448-7888   Fax: 1-631-938-8127  Europe: 44-207-048-3343   Email: info@creative-biomart.com
Home > Products > Assay Kits > Metabolism Kits > Bile Acid Assay Kit

Bile Acid Assay Kit

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Cat.No.:
Kit-2070
Product Overview:
Bile Acid Assay Kit provides a convenient fluorimetric means to measure total bile acids in biological samples. In the assay, 3α- hydroxysteroid dehydrogenase reacts with all twelve bile acids, converting NAD to NADH, which reduces a probe to a highly fluorescent product. The resulting fluorescence intensity (λexc/em = 530/585 nm) is linear to the bile acid concentration in the sample.
Size:
100 tests
Description:
Twelve different types of bile acids are typically found in mammals, among them two primary types are cholic acid and chenodeoxycholic acid. These can be dehydroxylated into secondary bile acids. Finally, these four can be conjugated to either taurine or glycine creating 8 different conjugated bile acids. Bile acid levels in feces, blood, urine, and bile can be used as markers for various diseases such as hyperlipidemia, cholestasis, gall stones, colon cancer, etc. Bile acids also exist in a sulfate salt form known as bile acid-sulfates. Sulfation of bile acids increases their solubility and decreases intestinal absorption, thereby enhancing fecal and urinary excretion. This assay does not measure bile acid-sulfates, and measures only the twelve non-sulfated bile acids.
Applications:
For quantitative determination of total bile acids and evaluation of drug effects on bile acid metabolism.
Storage:
The kit is shipped on ice. Store all reagents at -20°C. Shelf life of 6 months after receipt.
Kit Components:
Assay Buffer: 10 mL Enzyme A: 120 µLNAD Solution: 1 mL Enzyme B: 120 µLProbe: 750 µL Standard: 120 µL
Detection method:
FL530/585nm
Compatible Sample Types:
Serum, plasma, urine and other biological samples.
Features & Benefits:
• Safe. Non-radioactive assay.• Sensitive and accurate. Linear detection range of 1 - 150 μM bile acids. • Convenient and high-throughput. Homogeneous "mix-incubate-measure" type assay. No wash and reagent transfer steps are involved. Can bereadily automated on HTS liquid handling systems for processing thousands of samples per day.
Assay time:
20 min
Sensitivity:
Detection Limit: 1 uM


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Optional requirements on this protein +Expand
C-fusion   N-fusion  Non-tagged
His   GST  Fc  Others
<1.0 eu/μg   <0.1 eu/μg  <0.01 eu/μg  Not required
Monomer Isolation   Dimer Isolation   Not required
>80% by SDS-PAGE   >90% by SDS-PAGE  >95% by SDS-PAGE  Others
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45-1 Ramsey Road, Shirley, NY 11967, USA
USA: 1-631-559-9269  1-631-448-7888
Europe: 44-207-048-3343
FAX: 1-631-938-8127
EMAIL: info@creative-biomart.com

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