Address: 45-1 Ramsey Road, Shirley, NY 11967, USA    USA: 1-631-559-9269  1-631-448-7888   Fax: 1-631-938-8127  Europe: 44-207-048-3343   Email: info@creative-biomart.com
Home > Products > Assay Kits > Metabolism Kits > Branched Chain Amino Acid Assay Kit (Leu, Ile, Val)

Branched Chain Amino Acid Assay Kit (Leu, Ile, Val)

   Share
"Amino Acid" Related Products.
Cat.No.:
Kit-0132
Product Overview:
Branched Chain Amino Acid (Leu, Ile, Val) Assay Kit is used for measuring the BCAA
Description:
The branched-chain amino acids or BCAA"s, refer to the amino acids with non-linear aliphatic side-chains, namely leucine, isoleucine and valine. These three essential amino acids make up approximately 1/3 of skeletal muscle in the human body. BCAA"s are currently used clinically to aid in the recovery of burn victims, as well as for strength supplementation for athletes. BCAA"s, primarily Leu, can stimulate insulin secretion. The BCAA"s have also been implicated in a wide range of other physiological effects. The Branched Chain Amino Acid (Leu, Ile, Val) Assay Kit provides a simple convenient means of measuring the BCAA"s in a variety of biological samples. The kit utilizes an enzyme assay in which BCAA is oxidatively deaminated, producing NADH which reduces the probe, generating a colored product (λmax = 450 nm). The Branched Chain Amino Acid (Leu, Ile, Val) Assay Kit measures BCAA"s in the range of 0 to 10 nmol per sample with a detection limit of ~0.2 nmol (~10 µM BCAA in sample). BCAA"s are present in serum ~ 0.1-0.4 mM each (~0.125-1.5 mM combined).
Target Species:
Mammals
Usage:
For research use only (RUO)
Storage:
Store the kit at -20°C, protect from light. Allow Assay Buffer to warm to room temperature before use. Briefly centrifuge vials prior to opening.
Kit Components:
BCAA Assay Buffer 25 mLBCAA Enzyme Mix (lyophilized) 1 vialWST Substrate Mix (lyophilized) 1 vialLeu Standard (1 μmol) 100 µL
Detection method:
Colorimetric
Compatible Sample Types:
Biological Fluid, Cell Culture Supernatant, Plasma, Serum, Tissue Culture Supernatant, Urine


Online Inquiry    
Note: There will be extra charge for optional service!
Optional requirements on this protein +Expand
C-fusion   N-fusion  Non-tagged
His   GST  Fc  Others
<1.0 eu/μg   <0.1 eu/μg  <0.01 eu/μg  Not required
Monomer Isolation   Dimer Isolation   Not required
>80% by SDS-PAGE   >90% by SDS-PAGE  >95% by SDS-PAGE  Others
Please input "biomart" as verification code.
Please review Creative Biomart's privacy policy for more information.
Price does not include shipping and packaging costs.Request quote for final price.

If you have any other questions, please call
+1-631-559-9269.

online inquiry

Welcome! For price inquiries, please feel free to contact us through the form below. We will get back to you as soon as possible.

45-1 Ramsey Road, Shirley, NY 11967, USA
USA: 1-631-559-9269  1-631-448-7888
Europe: 44-207-048-3343
FAX: 1-631-938-8127
EMAIL: info@creative-biomart.com

facebook twitter linkedin google plus google plus

Join 10 other subscribers

24x7
CUSTOMER SERVICE
Easy access to products and services you need from our library via powerful searching tools
Copyright © 2007 - 2016 Creative Biomart. All Rights Reserved.