This colorimetric assay is designed for inhibitor screening or kinetic assay of recombinant CD45 tyrosine phosphatase. Activity is determined using the pp60c-src negative regulatory phosphopeptide substrate, an efficient and physiologically relevant peptide substrate for CD45. The detection of free phosphate released is based on the classic Malachite green assay.
Colorimetric detection, HTS
Recombinant CD45, pp60c-src negative regulatory phosphopeptide substrate, assay buffer, BIOMOL GREEN detection reagent, phosphate standard, inhibitor, microplate and detailed instructions.