"GR" Related Products

CHO-K1 GR Protein Interaction Assay Kit

Cat.No.: Kit-1657
Product Overview: NHR Protein Interaction assays monitor the interaction of activated NHRs with Steroid Receptor Co-activator Peptides (SRCPs) using Enzyme Fragment Complementation. The cells are engineered to two complementary fragments of β-galactosidase: the smaller ProLink (PK) fragment is fused to the NHR target of interest, and the larger Enzyme Acceptor (EA) is fused to the SRCP. Activation of the NHR target induces interaction of the receptor with the SRCP, forcing complementation of PK and EA. Activity of the resulting fully formed β-galactosidase enzyme is detected using a chemiluminescent substrate. The cells have been modified to prevent long term propagation and expansion using a proprietary compound that has no apparent effect on assay performance.
Size: 48-well/96-well
Target Species: Human
Storage: Short term (2 weeks): Store in vapor phase of liquid N2
Kit Components: 0.6 x 10^6 cells per vial in 0.5 mL (kit contains cells, detection reagents and plate(s))

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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