Address: 45-1 Ramsey Road, Shirley, NY 11967, USA    USA: 1-631-559-9269  1-631-448-7888   Fax: 1-631-938-8127  Europe: 44-207-048-3343   Email: info@creative-biomart.com
Home > Products > Assay Kits > Cellular Kits > Caspase-6 Colorimetric Assay Kit

Caspase-6 Colorimetric Assay Kit

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Cat.No.:
Kit-0170
Product Overview:
Colorimetric assay kit for caspase-6 activity
Size:
100 assay
Description:
Activation of ICE-family proteases/caspases initiates apoptosis in mammalian cells. The Caspase-6 Colorimetric Assay Kit provides a simple and convenient means for assaying the activity of caspases that recognize the sequence VEID. The assay is based on spectrophotometric detection of the chromophore p-nitroanilide (pNA) after cleavage from the labeled substrate VEID-pNA. The pNA light emission can be quantified using a spectrophotometer or a microtiter plate reader at 400-or 405-nm.
Applications:
Detect early/middle stages of apoptosis; differentiate apoptosis from necrosis.
Target Species:
Mammal
Usage:
For research use only. Not intended for any diagnostic or therapeutic purpose.
Storage:
Store at -20°C. Product has an expected shelf life of 6-12 months.
Kit Components:
Cell Lysis Buffer;2X Reaction Buffer;VEID-pNA (4 mM);DTT (1 M)Dilution Buffer;
Detection method:
Spectrophotometer (OD-400-or 405-nm)or a microtiter plate reader
Compatible Sample Types:
Cell and tissue lysates
Features & Benefits:
1. Simple one-step procedure; takes 1-2 hours.2. Fast and convenient.3. Comparison of the absorbance of pNA from an apoptotic sample with an uninduced control allows determination of the fold increase in caspase-6 activity


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Optional requirements on this protein +Expand
C-fusion   N-fusion  Non-tagged
His   GST  Fc  Others
<1.0 eu/μg   <0.1 eu/μg  <0.01 eu/μg  Not required
Monomer Isolation   Dimer Isolation   Not required
>80% by SDS-PAGE   >90% by SDS-PAGE  >95% by SDS-PAGE  Others
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45-1 Ramsey Road, Shirley, NY 11967, USA
USA: 1-631-559-9269  1-631-448-7888
Europe: 44-207-048-3343
FAX: 1-631-938-8127
EMAIL: info@creative-biomart.com

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