Simple procedure; takes ~ 30-40 minutes
Fast and convenient
Sensitive assays for measuring Catalase in various biological samples
Catalase (EC 126.96.36.199) is a ubiquitous antioxidant enzyme that is present in nearly all living organisms. It functions to catalyze the decomposition of hydrogen peroxide (H₂O₂) to water and oxygen. Catalase Assay Kit provides a highly sensitive, simple, direct and HTS-ready assay for measuring Catalase activity in biological samples. In the assay, catalase first reacts with H₂O₂ to produce water and oxygen, the unconverted H₂O₂ reacts with OxiRed probe to produce a product, which can be measured at 570 nm (Colorimetric method) or at Ex/Em=535/587nm (fluorometric method). Catalase activity is reversely proportional to the signal. The kit detects high pico-unit of catalase in samples.
The kit detects high pico-unit of catalase in samples.
For Research Use Only! Not For Use in Humans
• Catalase Assay Buffer
• OxiRed Probe (in DMSO)
• HRP (lyophilized)
• H₂O₂ (3%; 0.88M)
• Stop Solution
• Catalase Positive Control (lyophilized)
Absorbance (570 nm) or Fluorescence (Ex/Em 535/587 nm)
Compatible Sample Types:
Cell and Tissue culture supernatants, urine, plasma and serum, as well as many other biological fluids, growth medium, food etc.