Safe and non-radioactive assayConvenient 1-step detectionExcellent sensitivity
A complete colorimetric assay kit for measuring cellular calcineurin (PP2B) phosphatase activity. It employs a convenient 96-well plate format with all reagents necessary for measuring calcineurin (PP2B) phosphatase activity in tissue/cellular extracts PLUS human recombinant calcineurin is included as a positive control. The RII phosphopeptide substrate, supplied with this kit is the most efficient and selective peptide known for calcineurin. The detection of free-phosphate released is based on the classic malachite green assay.
Calcineurin enzyme, Calmodulin (human, recombinant), Substrate (RII phosphopeptide), 2x assay buffer, EGTA Buffer, Lysis Buffer, Protease inhibitor cocktail, Biomol Green Reagent, Phosphate standard, Okadaic acid, Desalting column and resin, ½-Volume microplate