Choline/Acetylcholine Quantification Kit is used for quantifying choline and acetylcholine by either a colorimetric or fluorometric method.
Choline and acetylcholine play important roles in many biological processes. The Choline/Acetylcholine Quantification Kit provides a simple and sensitive means for quantifying Choline and Acetylcholine by either a colorimetric or fluorometric method. In the assay free choline is oxidized to betaine, via the intermediate betaine aldehyde. The reaction generates products which react with the Choline Probe to generate color (λ= 570 nm), and fluorescence (Ex/Em 535/587 nm). Acetylcholine can be converted to choline by addingacetylcholinesterase to the reaction. The kit can detect choline and acetylcholine (total choline – free choline) in various biological samples such as in blood, cells, culture media, fermentation media, etc. There is no need for pretreatment or purification of samples. The kit can detect 10 pmol~5 nmol of choline or acetylcholine.
For research use only (RUO)
Store kit at -20°C. Warm the assay buffer to room temperature before use. Briefly centrifuge vials before opening. Read the entire protocol before performing the assay.
Choline Assay Buffer 25 mlCholine Probe* 200 μlCholine Enzyme Mix 1 VialAcetylcholinesterase 1 VialCholine Standard (5 µmol) 1 VialNote: *The fluorometric assay is ~10 times more sensitive than the colorimetric assay. Use 0.4 µl of the probe per reaction to decrease background/increase detection sensitivity significantly.
Compatible Sample Types:
Biological Fluid, Cell Culture Supernatant, Plasma, Serum, Tissue Culture Supernatant, Urine