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Home > Products > Assay Kits > Activity Kits > Complex IV Human Enzyme Activity Microplate Assay Kit

Complex IV Human Enzyme Activity Microplate Assay Kit

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Cat.No.:
Kit-0243
Product Overview:
The Complex IV Human Enzyme Activity Microplate Assay Kit is used to determine the activity of cytochrome c oxidase in a human sample with greater speed and simplicity. The COX enzyme is immunocaptured within the wells of the microplate and activity is determined colorimetrically by following the oxidation of reduced cytochrome c by the absorbance change at 550 nm. Included in this kit for performance of the activity assay are buffer, detergent, substrate, and 96-well microplate with monoclonal antibody pre-bound to the wells of the plate, allowing for a stream-lined assay.
Size:
1 x 96 well plate
Applications:
Functional Studies
Target Species:
Reacts with: Cow, Human, PigDoes not react withMouse, Rat
Storage:
Reagent C should be aliquoted and stored at -20°C or preferably at-80°C. Tube 1, Detergent and the covered microplate should bestored at 4°C-DO NOT FREEZE.
Kit Components:
Components 96 tests96-well microplate (12 strips) 1 unitBuffer 1 x 10mlDetergent 1 x 1mlReagent C (Cytochrome c) 1 x 1ml
Detection method:
Colorimetric
Compatible Sample Types:
Cell culture extracts, Tissue


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Optional requirements on this protein +Expand
C-fusion   N-fusion  Non-tagged
His   GST  Fc  Others
<1.0 eu/μg   <0.1 eu/μg  <0.01 eu/μg  Not required
Monomer Isolation   Dimer Isolation   Not required
>80% by SDS-PAGE   >90% by SDS-PAGE  >95% by SDS-PAGE  Others
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45-1 Ramsey Road, Shirley, NY 11967, USA
USA: 1-631-559-9269  1-631-448-7888
Europe: 44-207-048-3343
FAX: 1-631-938-8127
EMAIL: info@creative-biomart.com

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