Address: 45-1 Ramsey Road, Shirley, NY 11967, USA    USA: 1-631-559-9269  1-631-448-7888   Fax: 1-631-938-8127  Europe: 44-207-048-3343   Email:
Home > Products > Assay Kits > Detection Kits > D-Fructose/D-Glucose Assay Kit
"D-Fructose/D-Glucose" Related Products.
Product Overview:
Enzymatic method for the determination of D-Fructose and D-Glucose (total sugars). Based on the spectrophotometric measurement of NADPH produced through the reactions, after addition of hexokinase (HK), phosphoglucose isomerase (PGI) and Glucose-6-phosphate dehydrogenase (G6PDH).
110 tests
D-Fructose and D-glucose occur widely in plant organisms. In foods, they occur mainly in honey, wine and beer, and a range of solid foodstuffs such as bread and pastries, chocolate and candies. In the wine industry, the content of Dfructose and D-glucose (total reducing sugars) is one of the key quality parameters; it represents the amount of sugar available for yeast fermentation.
This rapid and simple specific enzymatic method is used for the simultaneous determination of D-fructose and D-glucose in foodstuffs, pharmaceuticals, cosmetics and biological samples. The analysis of these sugars can also be performed separately. This kit can be used for the auto-analysis of total reducing sugars.
Kit Components:
Solution 1. Imidazole buffer (25 mL, 2 M, pH 7.6) plus MgCl2 (100 mM) and sodium azide (0.02 % w/v) as a preservative. Stable for 2 years at 4 °C. Solution 2. NADP+ (250 mg) plus ATP (500 mg) and PVP (120 mg). Stable for 2 years at -20 °C. Dissolve in 12 mL of distilled water, divide into appropriately sized aliquots and store in PP tubes at -20 °C between use (stable for 2 years) and keep cool during use. Suspension 3. Hexokinase (EC; 425 U/mL) and Glucose-6-P dehydrogenase (EC; 212 U/mL) in 3.2 M ammonium sulphate (2.25 mL). Stable for 2 years at 4 °C. Swirl bottle before use. Suspension 4. Phosphoglucose isomerase (EC; 1000 U/mL) in 3.2 M ammonium sulphate (2.25 mL). Stable for 2 years at 4 °C. Swirl bottle before use. Solution 5. D-Fructose/D-glucose standard solution (5 mL, 0.20 mg/mL of each sugar). Stable for 2 years at room temperature. This standard solution can be used when there is some doubt about the method accuracy
Detection method:
UV method
Compatible Sample Types:
Wine, beer, fruit juices, milk, dietetic foods, bread, jam, honey, ice-creams, fruit and vegetables, pharmaceuticals, cosmetics and biological samples.
Features & Benefits:
Rapid reactionsPrevention of tanins inhibition (PVPP included)Suitable for manual and micro volume formats
Reaction volume: 2.34 mLRange: 2-800 mg/LDetection limit: 0.66 mg/L

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Note: There will be extra charge for optional service!
Optional requirements on this protein +Expand
C-fusion   N-fusion  Non-tagged
His   GST  Fc  Others
<1.0 eu/μg   <0.1 eu/μg  <0.01 eu/μg  Not required
Monomer Isolation   Dimer Isolation   Not required
>80% by SDS-PAGE   >90% by SDS-PAGE  >95% by SDS-PAGE  Others
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45-1 Ramsey Road, Shirley, NY 11967, USA
USA: 1-631-559-9269  1-631-448-7888
Europe: 44-207-048-3343
FAX: 1-631-938-8127

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