"Glucose" Related Products

D-Glucose HK Assay Kit

Cat.No.: Kit-2059
Product Overview: Enzymatic method for the determination of D-Glucose. Based on the spectrophotometric measurement of NADPH produced though the reactions, after addition of hexokinase (HK) and glucose-6-phosphate dehydrogenase (G6PDH).
Size: 110 tests
Description: D-Glucose occurs widely in plants and animals. It is an essential component of carbohydrate metabolism and occurs frequently in the free form along with D-fructose and sucrose. However, the more important forms are those of di- (lactose, maltose, sucrose), tri-, oligo- and polysaccharides (dextrins, starch, cellulose). It is present in significant quantities in honey, wine and beer, and a range of solid foodstuffs such as bread and pastries, chocolate and candies. Measurement of D-glucose is extremely important in biochemistry and clinical analysis, as well as in food analysis; it is mostly determined along with other carbohydrates.
Applications: This rapid and simple specific enzymatic method is used for the determination of D-glucose in foodstuffs such as baking agents, diet beer and dietetic foods, as well as in pharmaceuticals, cosmetics and biological samples. The analysis of D-glucose in foodstuffs is normally performed in conjunction with D-fructose, maltose and sucrose.
Kit Components: Solution 1. Imidazole buffer (25 mL, 2 M, pH 7.6) plus MgCl2 (0.5 M), MgCl2 (100 mM) and sodium azide (0.02 % w/v) as a preservative. Stable for 2 years at 4 °C. Solution 2. NADP+ (150 mg) plus ATP (440 mg). Stable for 5 years at -20 °C. Dissolve in 12 mL of distilled water, divide into appropriately sized aliquots and store in PP tubes at -20 °C between use (stable for 2 years) and keep cool during use. Suspension 3. Hexokinase (EC 2.7.1.1; 425 U/mL) and glucose-6-P dehydrogenase (EC 1.1.1.49; 212 U/mL) in 3.2 M ammonium sulphate (2.25 mL). Stable for 2 years at 4 °C. Swirl bottle before use. Solution 4. D-Glucose standard solution (6 mL, 0.40 mg/mL) in 0.02% benzoic acid. Stable for 2 years at room temperature. This standard solution can be used when there is some doubt about the method accuracy.
Detection method: UV method
Compatible Sample Types: Wine, beer, fruit juices, milk, dietetic foods, bread, jam, honey, ice-creams, fruit and vegetables, pharmaceuticals, cosmetics and biological samples.
Features & Benefits: Rapid reactionsSuitable for manual and micro volume formats
Sensitivity: Reaction volume: 2.32 mLRange: 2-800 mg/LDetection limit: 0.66 mg

Online Inquiry

Note: There will be extra charge for optional service!
Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

Please input "biomart" as verification code. Please review Creative BioMart's privacy policy for more information


Price does not include shipping and packaging costs.Request quote for final price.

Price Inquiry

Welcome! For price inquiries, please feel free to contact us through the form below. We will get back to you as soon as possible.