Address: 45-1 Ramsey Road, Shirley, NY 11967, USA    USA: 1-631-559-9269  1-631-448-7888   Fax: 1-631-938-8127  Europe: 44-207-048-3343   Email: info@creative-biomart.com
Home > Products > Assay Kits > Activity Kits > FABP4 Inhibitor/Ligand Screening Assay Kit

FABP4 Inhibitor/Ligand Screening Assay Kit

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GST(2)
His(10)
MYC/DDK(1)
N/A(2)
Cat.No.:
Kit-1954
Size:
1 ea
Description:
Fatty acid binding protein 4 (Adipocyte-FABP, aP2, FABP4) is one of nine known cytosolic fatty acid binding proteins ranging in size from 14-15 kDa containing 127-132 amino acids. FABP4 is highly expressed in adipocytes and is regulated by peroxisome-proliferator-activated receptor-γ (PPARγ) agonists, insulin and fatty acids. Recent studies using FABP4 gene deletion in mice indicate a dominant role for FABP4 in several chronic metabolic diseases. Therefore, inhibiting the function of FABP4 is a potential mechanism for the treatment of metabolic diseases like diabetes and atherosclerosis. The FABP4 Ligand Binding Assay Kit provides a simple, reproducible, and sensitive tool for the identification of FABP4 ligands. The assay makes use of a Detection Reagent that exhibits increased fluorescence at 500 nm when bound to FABP4. Any strong ligand and/or inhibitor of FABP4 will displace the Detection Reagent thereby reducing the fluorescence. FABP4 is provided in high purity and in sufficient quantity for 100 tests.
Storage:
-80°C
Kit Components:
Arachidonic Acid Control: 1 vial/200 μl; -80°C FABP Assay Buffer (10X): 1 vial/2 ml; -20°C FABP Assay Detection Reagent (5X): 1 vial/1 ml; -20°CFABP4 (human recombinant) Assay Reagent (5X): 1 vial/1 ml; -80°C 96-Well Solid Plate (black): 2 plates Room; temperature Foil Plate Covers: 2 covers Room; temperature


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Optional requirements on this protein +Expand
C-fusion   N-fusion  Non-tagged
His   GST  Fc  Others
<1.0 eu/μg   <0.1 eu/μg  <0.01 eu/μg  Not required
Monomer Isolation   Dimer Isolation   Not required
>80% by SDS-PAGE   >90% by SDS-PAGE  >95% by SDS-PAGE  Others
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45-1 Ramsey Road, Shirley, NY 11967, USA
USA: 1-631-559-9269  1-631-448-7888
Europe: 44-207-048-3343
FAX: 1-631-938-8127
EMAIL: info@creative-biomart.com

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