Address: 45-1 Ramsey Road, Shirley, NY 11967, USA    USA: 1-631-559-9269  1-631-448-7888   Fax: 1-631-938-8127  Europe: 44-207-048-3343   Email: info@creative-biomart.com
Home > Products > Assay Kits > Activity Kits > Factor VIIIa Activity Fluorometric Assay Kit

Factor VIIIa Activity Fluorometric Assay Kit

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Cat.No.:
Kit-0336
Product Overview:
Convenient, and rapid
The assay can detect activity as low as 1 ng of FVIIIa in a variety of samples.
Description:
The coagulation Factor VIII, also known as anti-hemophilic factor (AHF) is a vital blood-clotting protein. Factor VIII circulates in the bloodstream as an inactive protein, bound to a large multimeric glycoprotein called von Willebrand factor (VWF). Upon an injury to the blood vessel, VWF dissociates from FVIII and releases the active form of FVIII (FVIIIa). In the presence of calcium ions and negatively charged membrane phospholipids, activated factor VIII (FVIIIa) then binds to the activated Factor IX (FIXa) and proteolytically activates factor X (FX) to factor Xa (FXa). Factor VIIIa Activity Assay kit is based on the ability of FVIIIa to generate FXa. The generated FXa proteolytically cleaves a synthetic substrate and releases a fluorophore, AMC, which can be easily quantified by fluorescence microplate reader. The assay is simple, rapid and can detect activity as low as 1 ng of FVIIIa in a variety of samples.
Applications:
Determine activity of pure FVIIIa-Detect activity of FVIIIa in plasma, and serum
Usage:
For Research Use Only! Not For Use in Humans.
Storage:
-20°C
Size:
100 assays
Kit Components:
• FVIIIa Assay Buffer
• FXa Substrate-AMC
• Enzyme Mix I
• Enzyme Mix II
• Phospholipids
• FVIIIa Enzyme Standard (2.6 µg)
Detection method:
Fluorescence (Ex/Em = 360/450 nm)
Compatible Sample Types:
• Purified enzyme
• Serum, plasma


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Note: There will be extra charge for optional service!
Optional requirements on this protein +Expand
C-fusion   N-fusion  Non-tagged
His   GST  Fc  Others
<1.0 eu/μg   <0.1 eu/μg  <0.01 eu/μg  Not required
Monomer Isolation   Dimer Isolation   Not required
>80% by SDS-PAGE   >90% by SDS-PAGE  >95% by SDS-PAGE  Others
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45-1 Ramsey Road, Shirley, NY 11967, USA
USA: 1-631-559-9269  1-631-448-7888
Europe: 44-207-048-3343
FAX: 1-631-938-8127
EMAIL: info@creative-biomart.com

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