GGT Activity Assay Kit (Fluorometric) is a quantitative fluorometric determination of GGT activity.
The Gamma-Glutamyl Transferase (GGT; EC 220.127.116.11) is an enzyme that transfers gamma-glutamyl functional groups. It is found in many tissues, the most notable one being the liver, and has significance in medicine as a diagnostic marker. GGT Activity Assay Kit (Fluorometric) provides a convenient tool for sensitive detection of the GGT in a variety of samples. The GGT in sample will recognize L-γ-Glutamyl-AMC as a specific substrate leading to proportional fluorescence development. The activity of GGT can be easily quantified fluorometrically (Ex/Em = 365/460 nm). This assay detects GGT activity as low as 0.02 mIU in sample.
For research use only (RUO)
Store the kit at -20°C, protected from light. Allow Assay Buffer to warm to room temperature before use. Briefly centrifuge vials before opening. Read the entire protocol before performing the assay.
GGT Assay Buffer. Cap code: WM. 25 mlGGT Substrate. Cap code: NM. 1 BottleGGT Positive Control. Cap code: green. 1 VialAMC Standard (1mM). Cap code: yellow. 100 µl
Compatible Sample Types:
Cell Lysate, Serum, Tissue Lysate