Address: 45-1 Ramsey Road, Shirley, NY 11967, USA    USA: 1-631-559-9269  1-631-448-7888   Fax: 1-631-938-8127  Europe: 44-207-048-3343   Email: info@creative-biomart.com
Home > Products > GMP Proteins > GMP Recombinant Human GDNF Protein

GMP Recombinant Human GDNF Protein

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Cat.No.:
GDNF-133HG
Product Overview:
Recombinant Human GDNF, Arg109-Ile211, was produced in NS0 cell under cGMP guidelines.
Source:
NS0
Species:
Human
Form:
Lyophilized from a 0.2 μm filtered solution in PBS.
Bio-activity:
Measured in a cell proliferation assay using SH‑SY5Y human neuroblastoma cells.
The specific activity of recombinant human GDNF is approximately 3.1 x 10^3 units/μg, which is calibrated against recombinant human GDNF Reference Standard (NIBSC code: 09/266).
Measured by its binding ability in a functional ELISA. Immobilized Recombinant Human GFR alpha ‑1/GDNF R alpha ‑1 Fc Chimera at 1 µg/mL can bind Recombinant Human GDNF with an apparent Kd <1 nM.
Molecular Mass:
Predicted Molecular Mass: 11.6 (monomer) kDa
N-terminal Sequence Analysis:
Arg109-Gly-Gln-Arg-Gly-Lys-Asn-Arg-Gly-(Cys)
Endotoxin:
<1.0 EU per 1 μg of the protein by the LAL method.
Purity:
>97%, by SDS-PAGE with silver staining, under reducing conditions.
Storage:
Avoid repeated freeze-thaw cycles.
12 months, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
Reconstitution:
Reconstitute at 100 μg/mL in PBS.
Quality Statement:
Our GMP proteins are produced according to relevant sections of the following documents: WHO TRS, No. 822, 1992 Annex 1, Good Manufacturing Practices for Biological Products; USP Chapter 1043, Ancillary Materials for Cell, Gene and Tissue-Engineered Products and USP Chapter 92, Growth Factors and Cytokines Used in Cell Therapy Manufacturing.
Shipping:
The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.


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Optional requirements on this protein +Expand
C-fusion   N-fusion  Non-tagged
His   GST  Fc  Others
<1.0 eu/μg   <0.1 eu/μg  <0.01 eu/μg  Not required
Monomer Isolation   Dimer Isolation   Not required
>80% by SDS-PAGE   >90% by SDS-PAGE  >95% by SDS-PAGE  Others
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45-1 Ramsey Road, Shirley, NY 11967, USA
USA: 1-631-559-9269  1-631-448-7888
Europe: 44-207-048-3343
FAX: 1-631-938-8127
EMAIL: info@creative-biomart.com

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