Recombinant Human SHH, Cys24-Gly197, was produced in E. coli using non-animal reagents in an animal-free facility under cGMP guidelines.
Lyophilized from a 0.2 μm filtered solution in NaH2PO4, NaCl and DTT.
Measured by its ability to induce alkaline phosphatase production by C3H10T1/2 mouse embryonic fibroblast cells. Nakamura, T. et al. (1997) Biochem. Biophys. Res. Commun. 237:465. The ED50 for this effect is <5 μg/mL.
Predicted Molecular Mass: 20 kDa
SDS-PAGE: 22 kDa, reducing conditions
N-terminal Sequence Analysis:
<0.10 EU per 1 μg of the protein by the LAL method.
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Avoid repeated freeze-thaw cycles.
12 months, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
Reconstitute at 250 μg/mL in sterile deionized water.
Our GMP proteins are produced according to relevant sections of the following documents: WHO TRS, No. 822, 1992 Annex 1, Good Manufacturing Practices for Biological Products; USP Chapter 1043, Ancillary Materials for Cell, Gene and Tissue-Engineered Products and USP Chapter 92, Growth Factors and Cytokines Used in Cell Therapy Manufacturing.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.