Address: 45-1 Ramsey Road, Shirley, NY 11967, USA    USA: 1-631-559-9269  1-631-448-7888   Fax: 1-631-938-8127  Europe: 44-207-048-3343   Email:
Home > Products > Assay Kits > Activity Kits > Gluconokinase Activity Colorimetric Assay Kit

Gluconokinase Activity Colorimetric Assay Kit

"GntK" Related Products.
Product Overview:
Gluconokinase Activity Assay Kit (Colorimetric) is used to determine GntK activity at 450 nm.
Gluconokinase (GntK or ATP:D-gluconate 6-phosphotransferase or Gluconate Kinase; EC: is a key enzyme for Gluconate degradation pathway. In E. coli and yeast, Gluconokinase can convert gluconate into 6-Phosphate-D-Gluconate in an ATP dependent manner. Through Hexose Monophosphate Shunt (HMS) pathway, 6-Phosphate-D-Gluconate generates ribose-6-phosphate, which is critical for nucleotides and nucleic acid synthesis. Little is known of the mechanism of gluconate metabolism in humans despite its widespread use in medicine and consumer products. Gluconokinase Assay kit provides a quick and easy way for monitoring Gluconokinase activity in a variety of samples. In this kit, Gluconokinase converts Gluconate into 6-Phosphate-D-Gluconate in an ATP dependent manner. 6-Phosphate-D-Gluconate and ADP in turn undergoe a series of reactions to form an intermediate, which reacts with the probe to form a colored product with strong absorbance (OD 450 nm). The assay is simple, sensitive, and high-throughput adaptable. Detection limit:< 0.1mU.
Measurement of GntK in various samplesMechanistic study of Pentose Phosphate Pathway
For research use only (RUO)
Store the kit at -20°C, protected from light.
Kit Components:
GntK Assay Buffer 25 mLGntK Substrate 1 vialATP 1 vialGntK Converting Enzyme 1 vialGntK Probe 1 vialNADH Standard 1 vialGntK Positive Control 1 vial
Detection method:
Compatible Sample Types:
Prokaryote such as: E.coliAnimal tissues such as liver, kidney, etc.Adherent or suspension cells.

Online Inquiry    
Note: There will be extra charge for optional service!
Optional requirements on this protein +Expand
C-fusion   N-fusion  Non-tagged
His   GST  Fc  Others
<1.0 eu/μg   <0.1 eu/μg  <0.01 eu/μg  Not required
Monomer Isolation   Dimer Isolation   Not required
>80% by SDS-PAGE   >90% by SDS-PAGE  >95% by SDS-PAGE  Others
Please input "biomart" as verification code.
Please review Creative Biomart's privacy policy for more information.
Price does not include shipping and packaging costs.Request quote for final price.

If you have any other questions, please call

online inquiry

Welcome! For price inquiries, please feel free to contact us through the form below. We will get back to you as soon as possible.

45-1 Ramsey Road, Shirley, NY 11967, USA
USA: 1-631-559-9269  1-631-448-7888
Europe: 44-207-048-3343
FAX: 1-631-938-8127

facebook twitter linkedin google plus google plus

Join 10 other subscribers

Easy access to products and services you need from our library via powerful searching tools
Copyright © 2007 - 2017 Creative Biomart. All Rights Reserved.